Biomedical Microdevices

, Volume 12, Issue 4, pp 597–606

Combinatorial targeting and nanotechnology applications

  • Glauco R. Souza
  • Fernanda I. Staquicini
  • Dawn R. Christianson
  • Michael G. Ozawa
  • J. Houston Miller
  • Renata Pasqualini
  • Wadih Arap
Article

DOI: 10.1007/s10544-009-9340-6

Cite this article as:
Souza, G.R., Staquicini, F.I., Christianson, D.R. et al. Biomed Microdevices (2010) 12: 597. doi:10.1007/s10544-009-9340-6

Abstract

The development of improved methods for targeted cell detection is of general interest in many fields of research and drug development. There are a number of well-established techniques for the study and detection of biomarkers expressed in living cells and tissues. Many of them rely on multi-step procedures that might not meet ideal assay requirements for speed, cost, sensitivity, and specificity. Here we report and further validate an approach that enables spontaneous molecular assembly to generate biologically active networks of bacteriophage (phage) assembled with gold (Au) nanoparticles (termed Au-phage nanoshuttles). Here, the nanoshuttles preserve the cell binding and internalization attributes mediated by a displayed peptide targeted to a cell surface receptor. The organization of such targeted assemblies can be further manipulated to be used as a mutimodal detection assembly, and they can be characterized as fractal nanostructures by angle-dependent light scattering fractal dimension analysis. Targeted Au-phage nanoshuttles offer multiple functionalities for nanotechnology-based sensing and reporting, including enhanced florescence and improved contrast for darkfield microscopy.

Keywords

Combinatorial targeting Nanotechnology Nanoshuttles Multimodal detection Gold nanoparticle Phage Peptide Fractal Fluorescence Scattering Direct assembly Angle-dependent Light scattering Au-phage ELISA 

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Glauco R. Souza
    • 1
  • Fernanda I. Staquicini
    • 1
  • Dawn R. Christianson
    • 1
  • Michael G. Ozawa
    • 1
  • J. Houston Miller
    • 2
  • Renata Pasqualini
    • 1
  • Wadih Arap
    • 1
  1. 1.M. D. Anderson Cancer CenterThe University of TexasHoustonUSA
  2. 2.Department of ChemistryThe George Washington UniversityWashingtonUSA

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