Biomedical Microdevices

, Volume 11, Issue 4, pp 837–842

A soft and flexible biosensor using a phospholipid polymer for continuous glucose monitoring

Authors

  • MingXing Chu
    • Graduate School of Medical and Dental SciencesTokyo Medical and Dental University
  • Hiroyuki Kudo
    • Department of Biomedical Devices and Instrumentation, Institute of Biomaterials and BioengineeringTokyo Medical and Dental University
  • Takayuki Shirai
    • Graduate school, Tokyo University of Technology
  • Kumiko Miyajima
    • Department of Biomedical Devices and Instrumentation, Institute of Biomaterials and BioengineeringTokyo Medical and Dental University
  • Hirokazu Saito
    • Department of Biomedical Devices and Instrumentation, Institute of Biomaterials and BioengineeringTokyo Medical and Dental University
    • Department of Mechanical EngineeringTokyo National College of Technology
  • Nobuyuki Morimoto
    • Department of Organic Materials, Institute of Biomaterials and BioengineeringTokyo Medical and Dental University
    • Department of Materials Processing, Graduate School of EngineeringTohoku University
  • Kazuyoshi Yano
    • School of BionicsTokyo University of Technology
  • Yasuhiko Iwasaki
    • Department of Chemistry and Materials Engineering Faculty of ChemistryMaterials and Bioengineering Kansai University
  • Kazunari Akiyoshi
    • Department of Organic Materials, Institute of Biomaterials and BioengineeringTokyo Medical and Dental University
    • Department of Biomedical Devices and Instrumentation, Institute of Biomaterials and BioengineeringTokyo Medical and Dental University
Article

DOI: 10.1007/s10544-009-9300-1

Cite this article as:
Chu, M., Kudo, H., Shirai, T. et al. Biomed Microdevices (2009) 11: 837. doi:10.1007/s10544-009-9300-1

Abstract

A flexible biosensor using a phospholipid polymer to immobilization of glucose oxidase (GOD) was fabricated and tested. At first, an enzyme membrane formed by immobilizing GOD onto a porous polytetrafluoroethylene (PTFE) membrane using the phospholipid polymer (2-methacryloyloxyethyl phosphorylcholine (MPC) copolymerized with 2-ethylhexylmethacrylate (EHMA) : PMEH) was evaluated. According to the result of amperometric measurement, average density of GOD to be immobilized was optimized to 38.9 units cm−2. Temperature and pH dependences were also investigated. Then, a flexible glucose sensor was fabricated by immobilizing GOD onto a flexible hydrogen peroxide electrode using PMEH. The flexible glucose sensor showed a linear relationship between output currents and glucose concentration in 0.05–1.00 mmol L−1, with a correlation coefficient of 0.999. The calibration range covered the normal tear glucose level of 0.14–0.23 mmol L−1. This indicates that the flexible biosensor is considered to be useful for monitoring of glucose in tear fluids.

Keywords

BiosensorPhospholipid polymerFlexibleGlucose sensor

Copyright information

© Springer Science+Business Media, LLC 2009