Abstract
A flexible biosensor using a phospholipid polymer to immobilization of glucose oxidase (GOD) was fabricated and tested. At first, an enzyme membrane formed by immobilizing GOD onto a porous polytetrafluoroethylene (PTFE) membrane using the phospholipid polymer (2-methacryloyloxyethyl phosphorylcholine (MPC) copolymerized with 2-ethylhexylmethacrylate (EHMA) : PMEH) was evaluated. According to the result of amperometric measurement, average density of GOD to be immobilized was optimized to 38.9 units cm−2. Temperature and pH dependences were also investigated. Then, a flexible glucose sensor was fabricated by immobilizing GOD onto a flexible hydrogen peroxide electrode using PMEH. The flexible glucose sensor showed a linear relationship between output currents and glucose concentration in 0.05–1.00 mmol L−1, with a correlation coefficient of 0.999. The calibration range covered the normal tear glucose level of 0.14–0.23 mmol L−1. This indicates that the flexible biosensor is considered to be useful for monitoring of glucose in tear fluids.
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References
T. Adrega, D.M.F. Prazeres, V. Chu, J.P. Conde, J. Non-Cryst. Solids 352, 1999–2003 (2006). doi:10.1016/j.jnoncrysol.2006.01.079
S. Ahmed, C. Dack, G. Farace, G. Rigby, P. Vadgama, Anal. Chim. Acta 537, 153–161 (2005). doi:10.1016/j.aca.2005.01.065
Y.L. Bang, K.D. Song, B.S. Joo, J.S. Huh, S.D. Choi, D.D. Lee, Sensors Actuators B 102, 20–26 (2004). doi:10.1016/j.snb.2003.11.039
D.D. Borole, U.R. Kapadi, P.P. Mahulikar, D.G. Hundiwale, Eur. Polym. J. 41, 2183–2188 (2005). doi:10.1016/j.eurpolymj.2005.03.013
K.M. Daum, R.M. Hill, Invest. Ophthalmol. Vis. Sci 22, 509–514 (1982)
F.H. Fischer, M. Wiederholt, Graefes Arch. Clin. Exp. Ophthalmol 218, 168–170 (1982). doi:10.1007/BF02215658
M. Gerritsen, A. Kros, V. Sprakel, J.A. Lutterman, R.J.M. Nolte, J.A. Jansen, Biomaterials 21, 71–78 (2000). doi:10.1016/S0142-9612(99)00136-2
R. Gifford, J.J. Kehoe, S.L. Barnes, B.A. Kornilayev, M.A. Alterman, G.S. Wilson, Biomaterials 27, 2587–2598 (2006). doi:10.1016/j.biomaterials.2005.11.033
K. Ishihara, H. Nomura, T. Mihara, K. Kurita, Y. Iwasaki, N. Nakabayashi, J. Biomed. Mater. Res. 39(2), 323–330 (1998). doi:10.1002/(SICI)1097-4636(199802)39:2<323::AID-JBM21>3.0.CO;2-C
Z. Jin, R. Chen, L.A. Colon, Anal. Chem 69, 1326–1331 (1997). doi:10.1021/ac960724m
Z.H. Lam, K.S. Hwang, J.Y. Lee, J.G. Chase, G.C. Wake, Med. Eng. Phys 24, 663–672 (2002). doi:10.1016/S1350-4533(02)00147-9
C.Y. Li, J. Han, H. Chong, Biosens. Bioelectron 22, 1988–1993 (2007). doi:10.1016/j.bios.2006.08.043
J.K. Nielsen, C.B. Djurhuus, C.H. Gravholt, A.C. Carus, J. Granild-Jensen, H. Orskov, J.S. Christiansen, Diabetes 54, 1635–1639 (2005). doi:10.2337/diabetes.54.6.1635
A. Pasic, H. Koehler, I. Klimant, L. Schaupp, Sensors Actuators B 122, 60–68 (2007). doi:10.1016/j.snb.2006.05.010
C. Pedersen, S.T. Jespersen, K.W. Jacobsen, J.P. Krog, C. Christensen, E.V. Thomsen, Sensors Actuators A 115, 617–627 (2004). doi:10.1016/j.sna.2004.04.015
G. Piechotta, J. Albers, R. Hintsche, Biosens.Bioelectron 21, 802–808 (2005). doi:10.1016/j.bios.2005.02.008
F. Ricci, F. Caprio, A. Poscia, F. Valgimigli, D. Messeri, E. Lepori, G. Dall’Oglio, G. Palleschi, D. Moscone, Biosens. Bioelectron 22, 2032–2039 (2007). doi:10.1016/j.bios.2006.08.041
V. Sanz, S. Marcos, J. Galbán, Biosens. Bioelectron 22, 2876–2883 (2007). doi:10.1016/j.bios.2006.12.015
D.K. Sen, G.S. Sarin, Br. J. Ophthalmol 64, 693–695 (1980). doi:10.1136/bjo.64.9.693
D. Shan, M.J. Zhu, H.G. Xue, S. Cosnier, Biosens. Bioelectron 22, 1612–1617 (2007). doi:10.1016/j.bios.2006.07.019
H. Suzuki, H. Arakawa, I. Karube, Biosens. Bioelectron 16, 725–733 (2001). doi:10.1016/S0956-5663(01)00214-7
S.I. Taylor, Cell 97, 9–12 (1999). doi:10.1016/S0092-8674(00)80709-6
H. Zheng, H.G. Xue, Y.F. Zhang, Z.Q. Shen, Biosens. Bioelectron 17, 541–545 (2002). doi:10.1016/S0956-5663(02)00010-6
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This work was partly supported by Japan Society for the promotion of Science (JSPS) Grant-in-Aid for Scientific Research System and by MEXT (Ministry of Education, Culture, Sports, Science and Technology) Special Funds for Education and Research “Advanced Reasearch Program in Sensing Biology”.
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Chu, M., Kudo, H., Shirai, T. et al. A soft and flexible biosensor using a phospholipid polymer for continuous glucose monitoring. Biomed Microdevices 11, 837–842 (2009). https://doi.org/10.1007/s10544-009-9300-1
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DOI: https://doi.org/10.1007/s10544-009-9300-1