Article

Biomedical Microdevices

, Volume 11, Issue 4, pp 837-842

A soft and flexible biosensor using a phospholipid polymer for continuous glucose monitoring

  • MingXing ChuAffiliated withGraduate School of Medical and Dental Sciences, Tokyo Medical and Dental University
  • , Hiroyuki KudoAffiliated withDepartment of Biomedical Devices and Instrumentation, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University
  • , Takayuki ShiraiAffiliated withGraduate school, Tokyo University of Technology
  • , Kumiko MiyajimaAffiliated withDepartment of Biomedical Devices and Instrumentation, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University
  • , Hirokazu SaitoAffiliated withDepartment of Biomedical Devices and Instrumentation, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental UniversityDepartment of Mechanical Engineering, Tokyo National College of Technology
  • , Nobuyuki MorimotoAffiliated withDepartment of Organic Materials, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental UniversityDepartment of Materials Processing, Graduate School of Engineering, Tohoku University
  • , Kazuyoshi YanoAffiliated withSchool of Bionics, Tokyo University of Technology
  • , Yasuhiko IwasakiAffiliated withDepartment of Chemistry and Materials Engineering Faculty of Chemistry, Materials and Bioengineering Kansai University
  • , Kazunari AkiyoshiAffiliated withDepartment of Organic Materials, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University
    • , Kohji MitsubayashiAffiliated withDepartment of Biomedical Devices and Instrumentation, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University Email author 

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Abstract

A flexible biosensor using a phospholipid polymer to immobilization of glucose oxidase (GOD) was fabricated and tested. At first, an enzyme membrane formed by immobilizing GOD onto a porous polytetrafluoroethylene (PTFE) membrane using the phospholipid polymer (2-methacryloyloxyethyl phosphorylcholine (MPC) copolymerized with 2-ethylhexylmethacrylate (EHMA) : PMEH) was evaluated. According to the result of amperometric measurement, average density of GOD to be immobilized was optimized to 38.9 units cm−2. Temperature and pH dependences were also investigated. Then, a flexible glucose sensor was fabricated by immobilizing GOD onto a flexible hydrogen peroxide electrode using PMEH. The flexible glucose sensor showed a linear relationship between output currents and glucose concentration in 0.05–1.00 mmol L−1, with a correlation coefficient of 0.999. The calibration range covered the normal tear glucose level of 0.14–0.23 mmol L−1. This indicates that the flexible biosensor is considered to be useful for monitoring of glucose in tear fluids.

Keywords

Biosensor Phospholipid polymer Flexible Glucose sensor