Biomedical Microdevices

, Volume 11, Issue 4, pp 783–793

Novel biological/biohybrid prostheses for the ossicular chain: fabrication feasibility and preliminary functional characterization

Authors

    • CUCCS/RRMR (Centro per l’Uso Clinico delle Cellule Staminali/Rete Regionale di Medicina Rigenerativa)University of Pisa
    • Department of Oncology, Transplants and Advanced TechnologiesUniversity of Pisa
  • Cesare Stefanini
    • CRIM Lab (Center of Research In Microengineering)Scuola Superiore Sant’Anna
  • Delfo D’Alessandro
    • CUCCS/RRMR (Centro per l’Uso Clinico delle Cellule Staminali/Rete Regionale di Medicina Rigenerativa)University of Pisa
    • Department of Human Morphology & Applied BiologyUniversity of Pisa
  • Stefania Moscato
    • Department of Human Morphology & Applied BiologyUniversity of Pisa
  • Andrea Pietrabissa
    • Department of Oncology, Transplants and Advanced TechnologiesUniversity of Pisa
  • Mario Petrini
    • CUCCS/RRMR (Centro per l’Uso Clinico delle Cellule Staminali/Rete Regionale di Medicina Rigenerativa)University of Pisa
    • Department of Oncology, Transplants and Advanced TechnologiesUniversity of Pisa
  • Stefano Berrettini
    • Department of Neuroscience, ENT UnitUniversity of Pisa
Article

DOI: 10.1007/s10544-009-9293-9

Cite this article as:
Danti, S., Stefanini, C., D’Alessandro, D. et al. Biomed Microdevices (2009) 11: 783. doi:10.1007/s10544-009-9293-9

Abstract

Alternatives for ossicular replacements were fabricated in order to overcome persisting rejections in middle ear prosthetization. Unlike the synthetic prostheses in fashion, we propose biological and biohybrid replacements containing extra cellular matrix (ECM) molecules to improve biointegration. In this study, ECM-containing devices shaped as Partial Ossicular Replacement Prostheses (PORPs) were fabricated reproducing the current synthetic models. Biological PORPs were obtained from human decellularized cortical bone allografts by computer numerically controlled ultraprecision micromilling. Moreover, porous PORP-like scaffolds were produced and cultured with osteoinduced human mesenchymal stromal cells to generate in vitro bone ECM within the scaffold porosity (biohybrid PORPs). The acoustic responses of such devices were investigated and compared to those of commercial prostheses. Results showed that biological PORPs transmit mechanical signals with appropriate frequencies, amplitudes, and with early extinction time. Although signal transmission in biohybrid PORPs showed insufficient amplitude, we believe that tissue engineered constructs represent the new challenge in ossiculoplasty.

Keywords

Partial Ossicular Replacement Prosthesis (PORP)Middle earBone graftTissue engineeringMesenchymal Stem Cell (MSC)Acoustic features

Copyright information

© Springer Science+Business Media, LLC 2009