Biomedical Microdevices

, Volume 11, Issue 4, pp 723–729

Design of packed-fiber solid-phase extraction device for analysis of the drug and its metabolite in plasma

  • Xue-Jun Kang
  • Li-Qin Chen
  • Yan Wang
  • Yi-Yun Zhang
  • Zhong-Ze Gu
Article

DOI: 10.1007/s10544-009-9285-9

Cite this article as:
Kang, X., Chen, L., Wang, Y. et al. Biomed Microdevices (2009) 11: 723. doi:10.1007/s10544-009-9285-9

Abstract

A mini-column packed with 1 mg electrospun polystyrene nanofibers (about 200∼400 nm in diameter) was designed for simple, fast extraction of drugs, diazepam and its major metabolite, N-desmethyldiazepam for the analysis of them in human and dog plasma. Ttrezodone was selected as internal standard. The drugs adsorbed on the solid phase could be desorpted with 50 μl of the methanol and then monitored by liquid chromatography coupled to an ultraviolet detector. Parameters influencing the extraction efficiency such as fiber packing amount, eluted solvent, and pH of the sample were decided. The time for the pretreatment of 0.5 ml plasma sample was less than 10 min. The detection limits of diazepam and N-desmethyldiazepam in plasma could be as low as 1 μg/L. The intra- and inter-day precision, calculated from quality control (QC) samples, was less than 9.1%. The method was evaluated by its application in determination of dog plasma samples from three beagles after a single dose oral of diazepam. The technique was validated by comparison with conventional plasma analysis. It was observed that the mini-column offers improved limits of detection and reduced sample preparation time as compared to conventional method. For its simplicity and sensitivity, the method may be used in therapeutic drug monitoring and pharmacology study.

Keywords

Packed-fiber solid-phase extractionPreconcentrationNanofiberDiazepamHPLC-UV

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Xue-Jun Kang
    • 1
  • Li-Qin Chen
    • 1
  • Yan Wang
    • 1
  • Yi-Yun Zhang
    • 2
  • Zhong-Ze Gu
    • 2
  1. 1.Key Laboratory of Child Development and Learning Science, Ministry of Education, Research Center for Learning ScienceSoutheast UniversityNanjingChina
  2. 2.State Key Laboratory of Molecular and Biomolecular ElectronicsSoutheast UniversityNanjingChina