Biomedical Microdevices

, Volume 11, Issue 2, pp 379–387

Polymer coating of paramagnetic particulates for in vivo oxygen-sensing applications

  • Edward Eteshola
  • Ramasamy P. Pandian
  • Stephen C. Lee
  • Periannan Kuppusamy
Article

DOI: 10.1007/s10544-008-9244-x

Cite this article as:
Eteshola, E., Pandian, R.P., Lee, S.C. et al. Biomed Microdevices (2009) 11: 379. doi:10.1007/s10544-008-9244-x

Abstract

Crystalline lithium phthalocyanine (LiPc) can be used to sense oxygen. To enhance biocompatibility/stability of LiPc, we encapsulated LiPc in Teflon AF (TAF), cellulose acetate (CA), and polyvinyl acetate (PVAc) (TAF, previously used to encapsulate LiPc, was a comparator). We identified water-miscible solvents that don’t dissolve LiPc crystals, but are solvents for the polymers, and encapsulated crystals by solvent evaporation. Oxygen sensitivity of films was characterized in vitro and in vivo. Encapsulation did not change LiPc oximetry properties in vitro at anoxic conditions or varying partial pressures of oxygen (pO2). EPR linewidth of encapsulated particles was linear with pO2, responding to pO2 changes quickly and reproducibly for dynamic measurements. Encapsulated LiPc was unaffected by biological oxidoreductants, stable in vivo for four weeks. Oximetry, stability and biocompatibility properties of LiPc films were comparable, but both CA and PVAc films are cheaper, and easier to fabricate and handle than TAF films, making them superior.

Keywords

OximetryElectron Paramagnetic ResonanceLithium pthalocyananineSpin probesEPR

Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Edward Eteshola
    • 3
    • 4
  • Ramasamy P. Pandian
    • 1
    • 2
    • 4
  • Stephen C. Lee
    • 3
    • 4
  • Periannan Kuppusamy
    • 1
    • 2
    • 4
  1. 1.Center for Biomedical EPR Spectroscopy and ImagingThe Ohio State UniversityColumbusUSA
  2. 2.Department of Internal MedicineThe Ohio State UniversityColumbusUSA
  3. 3.Department of Biomedical EngineeringThe Ohio State UniversityColumbusUSA
  4. 4.Davis Heart & Lung Research InstituteThe Ohio State UniversityColumbusUSA