Biomedical Microdevices

, Volume 11, Issue 2, pp 369–377

Three-dimensional axisymmetric flow-focusing device using stereolithography

Article

DOI: 10.1007/s10544-008-9243-y

Cite this article as:
Morimoto, Y., Tan, WH. & Takeuchi, S. Biomed Microdevices (2009) 11: 369. doi:10.1007/s10544-008-9243-y

Abstract

This paper describes a three-dimensional microfluidic axisymmetric flow-focusing device (AFFD) fabricated using stereolithography. Using this method, we can fabricate AFFDs rapidly and automatically without cumbersome alignment needed in conventional methods. The AFFDs are able to be fabricated reproducibly with a micro-sized orifice of diameter around 250 μm. Using this device, we are able to produce monodisperse water-in-oil (W/O) droplets with a coefficient of variation (CV) of less than 4.5%, W/O droplets with encapsulated microbes (CV < 4.9%) and oil-in-water (O/W) droplets (CV < 3.2%) without any surface modifications. The diameter of these droplets range from 54 to 244 μm with respect to the flow rate ratio of the fluids used; these results are in good agreement with theoretical behavior. For applications of the AFFD, we demonstrate that these devices can be used to produce double emulsions and monodisperse hydrogel beads.

Keywords

Stereolithography Monodisperse 3D microfluidic channel Encapsulation Hydrogel 

Supplementary material

10544_2008_9243_Fig1_ESM.gif (35 kb)
Supplementary 1

The image of an inlet/outlet port of our AFFDs. These ports are similar to the joints commonly used for connecting syringes and tubes. Ports connected to silicone tubes are shown in Fig 3(a) (GIF 34.9 kb).

10544_2008_9243_Fig1_ESM.eps (11.6 mb)
High resolution image file (EPS 11.6 mb)
10544_2008_9243_Fig2_ESM.gif (24 kb)
Supplementary 2

The roughness of the channel surface in the block fabricated by stereolithography. Roughness measured in the vertical direction of the device (Ra = 916 nm) is larger than that measured in the horizontal direction (Ra = 65 nm) because in stereolithography, layers are accumulated in the vertical direction (GIF 24.1 kb).

10544_2008_9243_Fig2_ESM.eps (3.8 mb)
High resolution image file (EPS 3.79 mb)

Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  1. 1.Center for International Research on MicroMechatronics (CIRMM), Institute of Industrial Science (IIS)The University of TokyoMeguro-kuJapan
  2. 2.PRESTO, Japan Science and Technology AgencyChiyoda-kuJapan

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