Biomedical Microdevices

, Volume 11, Issue 2, pp 359–367

A micro circulating PCR chip using a suction-type membrane for fluidic transport

  • Liang-Ju Chien
  • Jung-Hao Wang
  • Tsung-Min Hsieh
  • Ping-Hei Chen
  • Pei-Jer Chen
  • Da-Sheng Lee
  • Ching-Hsing Luo
  • Gwo-Bin Lee
Article

DOI: 10.1007/s10544-008-9242-z

Cite this article as:
Chien, LJ., Wang, JH., Hsieh, TM. et al. Biomed Microdevices (2009) 11: 359. doi:10.1007/s10544-008-9242-z

Abstract

A new micromachined circulating polymerase chain reaction (PCR) chip is reported in this study. A novel liquid transportation mechanism utilizing a suction-type membrane and three microvalves were used to create a new microfluidic control module to rapidly transport the DNA samples and PCR reagents around three bio-reactors operating at three different temperatures. When operating at a membrane actuation frequency of 14.29 Hz and a pressure of 5 psi, the sample flow rate in the microfluidic control module can be as high as 18 μL/s. In addition, an array-type microheater was adopted to improve the temperature uniformity in the reaction chambers. Open-type reaction chambers were designed to facilitate temperature calibration. Experimental data from infrared images showed that the percentage of area inside the reaction chamber with a thermal variation of less than 1°C was over 90% for a denaturing temperature of 94°C. Three array-type heaters and temperature sensors were integrated into this new circulating PCR chip to modulate three specific operating temperatures for the denaturing, annealing, and extension steps of a PCR process. With this approach, the cycle numbers and reaction times of the three separate reaction steps can be individually adjusted. To verify the performance of this circulating PCR chip, a PCR process to amplify a detection gene (150 base pairs) associated with the hepatitis C virus was performed. Experimental results showed that DNA samples with concentrations ranging from 105 to 102copies/μL can be successfully amplified. Therefore, this new circulating PCR chip may provide a useful platform for genetic identification and molecular diagnosis.

Keywords

PCR Molecular diagnosis Microfluidics Microheaters MEMS 

Nomenclature

2D

Two-dimensional

Au

Gold Bio-MEMS Bio-micro-electro-mechanical-systems

bp

Base pair

HCV

Hepatitis C virus

DNA

Deoxyribonucleic acid

EMV

Electromagnetic valve

IR

Infrared

PCR

Polymerase chain reaction

PDMS

Polydimethylsiloxane

Pt

Platinum

SEM

Scanning electron microscope

Ti

Titanium

UV

Ultra-violet

Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Liang-Ju Chien
    • 1
  • Jung-Hao Wang
    • 1
  • Tsung-Min Hsieh
    • 2
  • Ping-Hei Chen
    • 4
  • Pei-Jer Chen
    • 5
  • Da-Sheng Lee
    • 6
  • Ching-Hsing Luo
    • 2
  • Gwo-Bin Lee
    • 1
    • 3
  1. 1.Department of Engineering ScienceNational Cheng Kung UniversityTainanTaiwan
  2. 2.Department of Electrical EngineeringNational Cheng Kung UniversityTainanTaiwan
  3. 3.Medical Electronics and Device Technology CenterIndustrial Technology Research InstituteHsinchuTaiwan
  4. 4.Department of Mechanical EngineeringNational Taiwan UniversityTaipeiTaiwan
  5. 5.National Taiwan University HospitalNational Taiwan UniversityTaipeiTaiwan
  6. 6.Department of Energy and Refrigerating Air-Conditioning EngineeringNational Taipei University of TechnologyTaipeiTaiwan

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