Article

Biomedical Microdevices

, Volume 10, Issue 6, pp 883-889

A uniaxial bioMEMS device for imaging single cell response during quantitative force-displacement measurements

  • David B. SerrellAffiliated withNational Institute of Standards and TechnologyUniversity of Colorado Email author 
  • , Jera LawAffiliated withNational Institute of Standards and Technology
  • , Andrew J. SlifkaAffiliated withNational Institute of Standards and Technology
  • , Roop L. MahajanAffiliated withVirginia Polytechnic Institute and State University
  • , Dudley S. FinchAffiliated withVirginia Polytechnic Institute and State University

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Abstract

A microfabricated device has been developed for imaging of a single, adherent cell while quantifying force under an applied displacement. The device works in a fashion similar to that of a displacement-controlled uniaxial tensile machine. The device was calibrated using a tipless atomic force microscope (AFM) cantilever and shows excellent agreement with the calculated spring constant. A step input was applied to a single, adherent fibroblast cell and the viscoelastic response was characterized with a mechanical model. The adherent fibroblast was imaged by use of epifluorescence and phase contrast techniques.

Keywords

Actin BioMEMS Fibroblasts Micro-fabricated Single-cell