A uniaxial bioMEMS device for imaging single cell response during quantitative force-displacement measurements
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- Serrell, D.B., Law, J., Slifka, A.J. et al. Biomed Microdevices (2008) 10: 883. doi:10.1007/s10544-008-9202-7
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A microfabricated device has been developed for imaging of a single, adherent cell while quantifying force under an applied displacement. The device works in a fashion similar to that of a displacement-controlled uniaxial tensile machine. The device was calibrated using a tipless atomic force microscope (AFM) cantilever and shows excellent agreement with the calculated spring constant. A step input was applied to a single, adherent fibroblast cell and the viscoelastic response was characterized with a mechanical model. The adherent fibroblast was imaged by use of epifluorescence and phase contrast techniques.