Biomedical Microdevices

, 10:883

A uniaxial bioMEMS device for imaging single cell response during quantitative force-displacement measurements

  • David B. Serrell
  • Jera Law
  • Andrew J. Slifka
  • Roop L. Mahajan
  • Dudley S. Finch
Article

DOI: 10.1007/s10544-008-9202-7

Cite this article as:
Serrell, D.B., Law, J., Slifka, A.J. et al. Biomed Microdevices (2008) 10: 883. doi:10.1007/s10544-008-9202-7

Abstract

A microfabricated device has been developed for imaging of a single, adherent cell while quantifying force under an applied displacement. The device works in a fashion similar to that of a displacement-controlled uniaxial tensile machine. The device was calibrated using a tipless atomic force microscope (AFM) cantilever and shows excellent agreement with the calculated spring constant. A step input was applied to a single, adherent fibroblast cell and the viscoelastic response was characterized with a mechanical model. The adherent fibroblast was imaged by use of epifluorescence and phase contrast techniques.

Keywords

Actin BioMEMS Fibroblasts Micro-fabricated Single-cell 

Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • David B. Serrell
    • 1
    • 2
  • Jera Law
    • 1
  • Andrew J. Slifka
    • 1
  • Roop L. Mahajan
    • 3
  • Dudley S. Finch
    • 3
  1. 1.National Institute of Standards and TechnologyBoulderUSA
  2. 2.University of ColoradoBoulderUSA
  3. 3.Virginia Polytechnic Institute and State UniversityBlacksburgUSA

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