Biomedical Microdevices

, Volume 10, Issue 2, pp 251–257

Silicon-based microfilters for whole blood cell separation

  • Hong Miao Ji
  • Victor Samper
  • Yu Chen
  • Chew Kiat Heng
  • Tit Meng Lim
  • Levent Yobas
Article

DOI: 10.1007/s10544-007-9131-x

Cite this article as:
Ji, H.M., Samper, V., Chen, Y. et al. Biomed Microdevices (2008) 10: 251. doi:10.1007/s10544-007-9131-x

Abstract

This paper reports on the comparison analysis of four main types of silicon-based microfilter for isolation of white blood cells (WBCs) from red blood cells (RBCs) in a given whole blood. The microfilter designs, namely, weir, pillar, crossflow, and membrane, all impose the same cut-off size of 3.5 μm to selectively trap WBCs. Using human whole blood, the microfilters have been characterized and compared for their blood handling capacity, WBCs trapping efficiency and RBCs passing efficiency. Based on the experimental results, the crossflow microfilter is superior and can be integrated with downstream components for on-chip genomic analysis.

Keywords

MicrofluidicFilterCross-flowSiliconSeparationLab on a chip

Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Hong Miao Ji
    • 1
  • Victor Samper
    • 1
  • Yu Chen
    • 1
  • Chew Kiat Heng
    • 2
  • Tit Meng Lim
    • 3
  • Levent Yobas
    • 1
  1. 1.Institute of MicroelectronicsSingaporeSingapore
  2. 2.Department of PediatricsNational University of SingaporeSingaporeSingapore
  3. 3.Department of Biological SciencesNational University of SingaporeSingaporeSingapore