Article

Biomedical Microdevices

, Volume 10, Issue 2, pp 243-249

DC-Dielectrophoretic separation of biological cells by size

  • Yuejun KangAffiliated withDepartment of Mechanical Engineering, Vanderbilt University
  • , Dongqing LiAffiliated withDepartment of Mechanical Engineering, Vanderbilt University Email author 
  • , Spyros A. KalamsAffiliated withInfectious Diseases Unit, Department of Internal Medicine, Vanderbilt University Medical CenterDepartment of Microbiology and Immunology, Vanderbilt University Medical Center
  • , Josiane E. EidAffiliated withDepartment of Cancer Biology, Vanderbilt University Medical Center

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Abstract

DC-Dielectrophoresis (DC-DEP), the induced motion of the dielectric particles in a spatially non-uniform DC electric field, is applied to separate biological cells by size. The locally non-uniform electric field is generated by an insulating hurdle fabricated within a PDMS microchannel. The cells experience a negative DEP (accordingly a repulsive) force at the corners of the hurdle where the gradient of local electric-field strength is the strongest. The DC-DEP force acting on the cells is proportional to the cells’ size. Thus the moving cells deviate from the streamlines and the degree of deviation is dependent on the cell size. In this paper, we demonstrated by using this method that, combined with the electroosmotic flow, mixed biological cells of a few to tens of micrometers difference in diameter can be continuously separated into different collecting wells. For separating target cells of a specific size, all that is required is to adjust the voltage outputs of the electrodes.

Keywords

DC-Dielectrophoresis Cell separation Microfluidics Lab-on-a-chip