Biomedical Microdevices

, Volume 10, Issue 1, pp 117–121

Microfluidic environment for high density hepatocyte culture

  • Mimi Y. Zhang
  • Philip J. Lee
  • Paul J. Hung
  • Terry Johnson
  • Luke P. Lee
  • Mohammed R. K. Mofrad
Article

DOI: 10.1007/s10544-007-9116-9

Cite this article as:
Zhang, M.Y., Lee, P.J., Hung, P.J. et al. Biomed Microdevices (2008) 10: 117. doi:10.1007/s10544-007-9116-9

Abstract

We present a microfluidic bioreactor for culturing high-density arrays of hepatocytes in a tissue-like micro-architecture. The microfluidic environment mimicked physiological liver mass transport, enabling sustained culture of high density cells (>2,000 cells/mm2) without nutrient limitation for over 1 week. The key feature of this design was a microporous microfluidic barrier that formed a sieved-pocket to concentrate cells during loading. Nutrient depletion within the cell mass was avoided by maintaining a continuous flow of medium (10 μl/day) that diffused across the porous barrier. Human hepatoma cells (HepG2/C3A) remained viable and functional as demonstrated by fluorescent viability assays and secretion of albumin for the one-week culture period.

Keywords

Microfluidics Cell culture Hepatocytes Bioreactor 

Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Mimi Y. Zhang
    • 2
  • Philip J. Lee
    • 1
  • Paul J. Hung
    • 1
  • Terry Johnson
    • 2
  • Luke P. Lee
    • 2
  • Mohammed R. K. Mofrad
    • 2
  1. 1.CellASIC CorporationSan LeandroUSA
  2. 2.UCSF/UC Berkeley Joint Graduate Group in BioengineeringBerkeleyUSA