Biomedical Microdevices

, Volume 9, Issue 4, pp 611–617

Lectin-modified microchannels for mammalian cell capture and purification

  • Ting Zheng
  • Hongmei Yu
  • Caroline M. Alexander
  • David J. Beebe
  • Lloyd M. Smith
Article

DOI: 10.1007/s10544-007-9074-2

Cite this article as:
Zheng, T., Yu, H., Alexander, C.M. et al. Biomed Microdevices (2007) 9: 611. doi:10.1007/s10544-007-9074-2
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Abstract

There is a need for cell purification strategies suitable for handling small cell populations (tens to thousands of cells). We present here a novel strategy for small scale cell purifications using lectin-modified microchannels. Mammalian cells can be selectively captured in lectin-modified channels and eluted with a solution of the lectin’s inhibiting sugar. As a proof-of-principle demonstration, two cell lines with different binding affinities to the lectin Sambucus nigra agglutinin (SNA) were combined at various ratios and introduced into SNA-modified microchannels. After incubation and washing, enrichments of approximately ten-fold were obtained for the SNA-binding cell type. The results demonstrate the potential of this miniaturized device for manipulation and purification of limited quantities of cells.

Keywords

Microfluidics Microchannels Lectins Cell purification 

Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Ting Zheng
    • 1
  • Hongmei Yu
    • 2
  • Caroline M. Alexander
    • 3
  • David J. Beebe
    • 2
  • Lloyd M. Smith
    • 1
  1. 1.Department of ChemistryUniversity of Wisconsin-MadisonMadisonUSA
  2. 2.Department of Biomedical EngineeringUniversity of Wisconsin-MadisonMadisonUSA
  3. 3.Department of OncologyUniversity of Wisconsin-MadisonMadisonUSA

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