Biomedical Microdevices

, Volume 9, Issue 6, pp 911–922

Design and fabrication of an artificial cornea based on a photolithographically patterned hydrogel construct

Authors

  • David Myung
    • Department of OphthalmologyStanford University School of Medicine
    • Department of Chemical EngineeringStanford University
  • Wongun Koh
    • Department of Chemical EngineeringYonsei University
  • Amit Bakri
    • Department of OphthalmologyStanford University School of Medicine
  • Fan Zhang
    • Department of BioengineeringStanford University
  • Amanda Marshall
    • Department of Chemical EngineeringStanford University
  • Jungmin Ko
    • Department of Chemical EngineeringStanford University
  • Jaan Noolandi
    • Department of OphthalmologyStanford University School of Medicine
    • Department of Chemical EngineeringStanford University
  • Michael Carrasco
    • Department of ChemistrySanta Clara University
  • Jennifer R. Cochran
    • Department of BioengineeringStanford University
  • Curtis W. Frank
    • Department of Chemical EngineeringStanford University
    • Department of OphthalmologyStanford University School of Medicine
    • Department of OphthalmologyStanford University
Article

DOI: 10.1007/s10544-006-9040-4

Cite this article as:
Myung, D., Koh, W., Bakri, A. et al. Biomed Microdevices (2007) 9: 911. doi:10.1007/s10544-006-9040-4

Abstract

We describe the design and fabrication of an artificial cornea based on a photolithographically patterned hydrogel construct, and demonstrate the adhesion of corneal epithelial and fibroblast cells to its central and peripheral components, respectively. The design consists of a central “core” optical component and a peripheral tissue-integrable “skirt.” The core is composed of a poly(ethylene glycol)/poly(acrylic acid) (PEG/PAA) double-network with high strength, high water content, and collagen type I tethered to its surface. Interpenetrating the periphery of the core is a microperforated, but resilient poly(hydroxyethyl acrylate) (PHEA) hydrogel skirt that is also surface-modified with collagen type I. The well-defined microperforations in the peripheral component were created by photolithography using a mask with radially arranged chrome discs. Surface modification of both the core and skirt elements was accomplished through the use of a photoreactive, heterobifunctional crosslinker. Primary corneal epithelial cells were cultured onto modified and unmodified PEG/PAA hydrogels to evaluate whether the central optic material could support epithelialization. Primary corneal fibroblasts were seeded onto the PHEA hydrogels to evaluate whether the peripheral skirt material could support the adhesion of corneal stromal cells. Cell growth in both cases was shown to be contingent on the covalent tethering of collagen. Successful demonstration of cell growth on the two engineered components was followed by fabrication of core-skirt constructs in which the central optic and peripheral skirt were synthesized in sequence and joined by an interpenetrating diffusion zone.

Keywords

Artificial corneaKeratoprosthesisPhotolithographyTissue integrationEpithelializationDouble-networkInterpenetrating networkHydrogel

Copyright information

© Springer Science+Business Media, LLC 2007