Biomedical Microdevices

, Volume 8, Issue 1, pp 65–71

Elucidating in vitro cell-cell interaction using a microfluidic coculture system


DOI: 10.1007/s10544-006-6384-8

Cite this article as:
Wei, CW., Cheng, JY. & Young, TH. Biomed Microdevices (2006) 8: 65. doi:10.1007/s10544-006-6384-8


This work presents a novel microfluidic coculture system that improves the accuracy of evaluating the interaction between cocultured cell types. A microfluidic coculture chip, fabricated by CO2 laser direct-writing on polymethyl methacrylate (PMMA), was designed to separate two cell types using a microchannel, while permitting transfer of cellular media. The system has two up-stream wells and five down-stream wells. As an example, released inflammatory cytokines (e.g., interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α)), activated in up-stream macrophages, flow through a microfluidic mixing system, generating linear concentration gradients in down-stream wells and inducing down-stream osteoblasts to release prostaglandin E2 (PGE2), a well-known bone resorption marker. Osteoblast viability was assessed by 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) assay. This novel coculture system can be applied to evaluate cell-cell interaction while physically separating interacting cells.


MicrofluidicCocultureCell-cell interaction

Copyright information

© Springer Science + Business Media, Inc. 2006

Authors and Affiliations

  1. 1.Research Center for Applied SciencesAcademia SinicaTaiwan
  2. 2.Institute of Biomedical EngineeringNational Taiwan UniversityTaiwan