Biochemistry (Moscow)

, Volume 70, Issue 10, pp 1086–1094

Computer-Aided Analysis of Spatial Structure of Some Hydrolytic Enzymes

  • V. G. Artyukhov
  • T. A. Kovaleva
  • O. M. Kozhokina
  • L. A. Bitutskaya
  • R. V. Dronov
  • O. D. Trofimova
Article

DOI: 10.1007/s10541-005-0229-6

Cite this article as:
Artyukhov, V.G., Kovaleva, T.A., Kozhokina, O.M. et al. Biochemistry (Moscow) (2005) 70: 1086. doi:10.1007/s10541-005-0229-6

Abstract

Using the MolScript version 2.1 computer program for protein molecule modeling and X-ray structure analysis data the spatial structures of several hydrolytic enzymes have been compared. These include glucoamylase from Aspergillus awamori and Saccharomycopsis fibuligera and lipases from Rhizopus japonicus. Results on homology of amino acid sequences and topology of secondary structure elements were obtained. 3D models of these enzymes with positioning of functionally important groups in the active site cavity were built.

Key words

enzymeamino acid sequencesecondary structurespatial modeltopologycomputer-aided analysis

Copyright information

© MAIK "Nauka/Interperiodica" 2005

Authors and Affiliations

  • V. G. Artyukhov
    • 1
  • T. A. Kovaleva
    • 1
  • O. M. Kozhokina
    • 1
  • L. A. Bitutskaya
    • 1
  • R. V. Dronov
    • 1
  • O. D. Trofimova
    • 1
  1. 1.Voronezh State UniversityVoronezhRussia