Biochemistry (Moscow)

, Volume 70, Issue 3, pp 330–338

Sialoside-binding macrophage lectins in phagocytosis of apoptotic bodies

Authors

  • E. M. Rapoport
    • Shemyakin and Ovchinnikov Institute of Bioorganic ChemistryRussian Academy of Sciences
  • Yu. B. Sapot’ko
    • Shemyakin and Ovchinnikov Institute of Bioorganic ChemistryRussian Academy of Sciences
  • G. V. Pazynina
    • Shemyakin and Ovchinnikov Institute of Bioorganic ChemistryRussian Academy of Sciences
  • V. K. Bojenko
    • Russian Research Center for Roentgen RadiologyRussian Ministry of Public Health
    • Shemyakin and Ovchinnikov Institute of Bioorganic ChemistryRussian Academy of Sciences
Article

DOI: 10.1007/s10541-005-0119-y

Cite this article as:
Rapoport, E.M., Sapot’ko, Y.B., Pazynina, G.V. et al. Biochemistry (Moscow) (2005) 70: 330. doi:10.1007/s10541-005-0119-y

Abstract

Elimination of apoptotic bodies is one of the important functions of macrophages. The aim of this work was to study the role of macrophage lectins in this process. Macrophage lectins were probed with neoglycoconjugates Glyc-PAA-fluo where carbohydrate is linked to fluorescein-labeled polyacrylamide (MW 30 kD). It was shown that neoglycoconjugates containing a Neu5Acα2-3Gal fragment bound to macrophages isolated from blood of healthy donors. Besides, carbohydrate chains containing the same fragment were revealed on apoptotic bodies. Phagocytosis of apoptotic bodies by macrophages was inhibited with sialooligosaccharide ligands of siglec-5 and MAbs to siglec-5. Thus, siglec-5 expressed on macrophages could participate in phagocytosis of apoptotic bodies. In addition, the role of siglecs in engulfment of apoptotic bodies by tumor-associated macrophages was studied. The phagocytic potency of macrophages isolated from blood of breast cancer patients was lower than engulfment ability of macrophages obtained from healthy donors and depended on tumor degree. Staining of macrophages obtained from blood of tumor patients with sialylated Glyc-PAA-fluo probes was more intense than that of macrophages from healthy donors; phagocytosis of apoptotic bodies by tumor associated macrophages was inhibited by carbohydrates that are known to be ligands for siglecs.

Key words

apoptosisbreast cancerglycoconjugatesphagocytosissiglecs

Abbreviations

FITC

fluorescein isothiocyanate

Glyc-PAA

polyacrylamide glycoconjugates

GM-CSF

granulocyte and macrophage colony-stimulating factor

fluo

fluorescein residue

PBS

phosphate-buffered saline containing 0.01 M Na2HPO4, 0.01 M NaH2PO4, 0.15 M NaCl (pH 7.3)

PBA

PBS supplemented with 0.2% BSA

SiaLex

Neu5Acα2-3Galβ1-4(Fucα1-3)GlcNAc

3′SiaLac

Neu5Acα2-3Galβ1-4Glc

6′SiaLac

Neu5Acα2-6Galβ1-4Glc

3′SiaTF

Neu5Acα2-3Galβ1-3GalNAcα

3′SiaLec

Neu5Acα2-3Galβ1-3GlcNAc

SiaTn

Neu5Acα2-6GalNAcα

6(Gal)OSuSiaLex

Neu5Acα2-3(6-HSO3)Galβ1-4(Fucα1-3)GlcNAc

6(GlcNAc)OSuSiaLex

Neu5Acα2-3Galβ1-4(Fucα1-3)(6-HSO3)GlcNAc

Sia2

Neu5Acα2-8Neu5Acα

Copyright information

© MAIK “Nauka/Interperiodica” 2005