Biologia Plantarum

, Volume 57, Issue 1, pp 56–62

Molecular cloning and expression analysis of a new stress-related AREB gene from Arachis hypogaea

  • L. Hong
  • B. Hu
  • X. Liu
  • C. Y. He
  • Y. Yao
  • X. L. Li
  • L. Li
Article

DOI: 10.1007/s10535-012-0236-6

Cite this article as:
Hong, L., Hu, B., Liu, X. et al. Biol Plant (2013) 57: 56. doi:10.1007/s10535-012-0236-6

Abstract

An AREB gene, designated as AhAREB1, was cloned from peanut (Arachis hypogaea L.). The gene contains a 1 338-bp open reading frame that encodes a putative protein of 445 amino acids. The corresponding genomic DNA containing four exons and three introns was isolated and analyzed. An upstream 1 060-bp DNA promoter fragment of the AhAREB1 gene was also amplified from peanut genomic DNA. Multiple sequence alignment of the deduced amino acids of AREB showed that the AhAREB1 protein shares high sequence homology with GmAREB1, S1AREB and ABF2. Quantitative real-time PCR analysis showed that AhAREB1 was induced by polyethylene glycol, NaCl, gibberellic acid, abscisic acid and salicylic acid. The cloning and characterization of the AhAREB1 gene will be useful for further studies establishing the biological role of AhAREB1 in plants.

Additional key words

ABAAhAREB1GA3peanutPEGSAwater stress

Abbreviations

ABA

abscisic acid

ABF

ABRE binding factor

ABRE

ABA response element

AREB

ABA-response element binding protein

bZIP

basic leucine zipper

CaMK

calmodulin-dependent protein kinase

CK

casein kinase

GA3

gibberellic acid

NCED

9-cis-epoxycarotenoid dioxygenase

ORF

open reading frame

PEG

polyethylene glycol

PKC

protein kinase C

SA

salicylic acid

W-BOX

WRKY binding site

WRKY

superfamily of plant transcription factors

Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • L. Hong
    • 1
    • 2
  • B. Hu
    • 1
  • X. Liu
    • 1
  • C. Y. He
    • 1
  • Y. Yao
    • 1
  • X. L. Li
    • 1
  • L. Li
    • 1
  1. 1.Guangdong Provincial Key Lab of Biotechnology for Plant Development, College of Life SciencesSouth China Normal UniversityGuangzhouP.R. China
  2. 2.College of Horticulture and Landscape ArchitectureZhongkai University of Agriculture and EngineeringGuangzhouP.R. China