Biologia Plantarum

, Volume 56, Issue 3, pp 451–457

Engineering ascorbic acid biosynthetic pathway in Arabidopsis leaves by single and double gene transformation

  • Y. Zhou
  • Q. C. Tao
  • Z. N. Wang
  • R. Fan
  • Y. Li
  • X. F. Sun
  • K. X. Tang
Article

DOI: 10.1007/s10535-012-0119-x

Cite this article as:
Zhou, Y., Tao, Q.C., Wang, Z.N. et al. Biol Plant (2012) 56: 451. doi:10.1007/s10535-012-0119-x

Abstract

Six genes, which encode enzymes involved in ascorbic acid (AsA) biosynthesis, including guanosine diphosphate (GDP)-mannose pyrophosphorylase (GMP), GDP-mannose-3′,5′-epimerase (GME), GDP-galactose guanylyltransferase (GGT), L-galactose-1-phosphate phosphatase (GPP), L-galactose dehydrogenase (GDH) and L-galactono-1,4-lactone dehydrogenase (GLDH) were transformed into Arabidopsis thaliana, to evaluate the contribution of each gene to AsA accumulation. Additionally, two combinations, GGT-GPP and GGT-GLDH, were co-transformed into Arabidopsis with a reliable double-gene transformation system. AsA content of GGT transgenic lines was 2.9-fold higher as compared to the control, and co-transformation led up to 4.1-fold AsA enhancement. These results provided further evidence that GGT is the key enzyme in plant AsA biosynthesis.

Additional key words

GDP-L-galactose guanyltransferasetransgenic plantsvitamin C

Abbreviations

GDH

L-galactose dehydrogenase

GDP

guanosine diphosphate

GGT

GDP-galactose guanylyltransferase

GLDH

L-galactono-1,4-lactone dehydrogenase

GME

GDP-mannose-3′,5′-epimerase

GMP

GDP-mannose pyrophosphorylase

GPP

L-galactose-1-phosphate phosphatase

Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Y. Zhou
    • 1
  • Q. C. Tao
    • 1
  • Z. N. Wang
    • 1
  • R. Fan
    • 1
  • Y. Li
    • 1
  • X. F. Sun
    • 1
  • K. X. Tang
    • 2
  1. 1.State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan-SJTU-Nottingham Plant Biotechnology R&D CenterFudan UniversityShanghaiP.R. China
  2. 2.Plant Biotechnology Research Center, School of Agriculture and Biology, Fudan-SJTU-Nottingham Plant Biotechnology R&D CenterShanghai Jiao Tong UniversityShanghaiP.R. China