, Volume 56, Issue 3, pp 451-457
Date: 03 Mar 2012

Engineering ascorbic acid biosynthetic pathway in Arabidopsis leaves by single and double gene transformation

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Six genes, which encode enzymes involved in ascorbic acid (AsA) biosynthesis, including guanosine diphosphate (GDP)-mannose pyrophosphorylase (GMP), GDP-mannose-3′,5′-epimerase (GME), GDP-galactose guanylyltransferase (GGT), L-galactose-1-phosphate phosphatase (GPP), L-galactose dehydrogenase (GDH) and L-galactono-1,4-lactone dehydrogenase (GLDH) were transformed into Arabidopsis thaliana, to evaluate the contribution of each gene to AsA accumulation. Additionally, two combinations, GGT-GPP and GGT-GLDH, were co-transformed into Arabidopsis with a reliable double-gene transformation system. AsA content of GGT transgenic lines was 2.9-fold higher as compared to the control, and co-transformation led up to 4.1-fold AsA enhancement. These results provided further evidence that GGT is the key enzyme in plant AsA biosynthesis.

Acknowledgments: We want to thank Mr. Y. Wang (Shanghai Jiaotong University, China) for the help with the instruction of HPLC analyses. This research is supported by China National High-Tech “863” Program (No. 2011AA100605), China Transgenic Research Program (No. 2011ZX08002-001) and the Shanghai Leading Academic Discipline Project (Np. B209).