Biologia Plantarum

, Volume 55, Issue 3, pp 431–436

Construction and characterization of a cDNA library from floral organs and fruitlets of Citrus reticulata

  • T. G. Zheng
  • W. M. Qiu
  • G. E. Fan
  • B. B. Zheng
  • W. W. Guo
Original Papers

DOI: 10.1007/s10535-011-0107-6

Cite this article as:
Zheng, T.G., Qiu, W.M., Fan, G.E. et al. Biol Plant (2011) 55: 431. doi:10.1007/s10535-011-0107-6

Abstract

To explore and isolate genes related to flowering and fruit development, we constructed a cDNA library from floral organs and fruitlets of Ponkan mandarin (Citrus reticulata Blanco). A total of 661 high-quality expressed sequence tags (ESTs) were generated and submitted to GenBank with the accession numbers from GO343532 to GO344192. All these ESTs were assembled into 43 contigs and 296 singletons (totally 339 unigenes). The BLAST2GO software was employed to annotate the unigenes, among which 77 ones had no significant homology with the sequences in NCBI non-redundant proteins database by BLASTX analysis. Additionally, gene ontology (GO) analysis revealed an overview of sequences distribution, which implied some specially expressed genes related to flower and fruit development. Furthermore, some abundantly expressed unigenes involved in several crucial metabolic pathways related to fruit quality were highlighted and three types of homologues of miraculin-like protein2 were analyzed by both semiquantitative RT-PCR and real-time PCR. The results showed different expression profiles of these genes, which meant that they contribute distinctly to fruit development.

Additional key words

expressed sequence tags Ponkan mandarin 

Abbreviations

EST

expressed sequence tags

NCBI

National Center for Biotechnology Information

ORF

open reading frame

RT-PCR

reverse transcription-polymerase chain reaction

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • T. G. Zheng
    • 1
  • W. M. Qiu
    • 1
  • G. E. Fan
    • 1
  • B. B. Zheng
    • 1
  • W. W. Guo
    • 1
  1. 1.Key Laboratory of Horticultural Plant Biology, Ministry of EducationHuazhong Agricultural UniversityWuhanChina

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