Biologia Plantarum

, Volume 55, Issue 2, pp 340–344

Effects of salt and osmotic stresses on free polyamine content and expression of polyamine biosynthetic genes in Vitis vinifera

Brief Communication

DOI: 10.1007/s10535-011-0050-6

Cite this article as:
Liu, J.H., Nakajima, I. & Moriguchi, T. Biol Plant (2011) 55: 340. doi:10.1007/s10535-011-0050-6

Abstract

Grape (Vitis vinifera L.) seedlings grown in vitro were treated with either 200 mM NaCl or 350 mM mannitol for 7 d. Both salinity and osmotic stress caused significant increase in electrolyte leakage. From the three commonly occurring free polyamines (PA), only conspicuous accumulation of putrescine was found in the NaCl-treated seedlings. Four PA biosynthetic genes encoding arginine decarboxylase (pVvADC), S-adenosylmethionine decarboxylase (pVvSAMDC), spermidine synthase (pVvSPDS) and spermine synthase (pVvSPMS) were successfully isolated. While induction of pVvADC was observed from the 1st day of salt treatment, pVvSAMDC and pVvSPMS were induced only at late stage of stress. As for expression levels of genes in the mannitol-treated seedling, either temporary (pVvADC at day 1) or late (pVvSPMS at days 5 and 7) induction was observed.

Additional key words

abiotic stresselectrolyte leakagegrapemannitolNaClputrescinesperminespermidine

Abbreviations

ADC

arginine decarboxylase

EL

electrolyte leakage

MS

Murashige and Skoog

ODC

ornithine decarboxylase

Put

putrescine

SAMDC

S-adenosylmethionine decarboxylase

Spd

spermidine

SPDS

spermidine synthase

Spm

spermine

SPMS

spermine synthase

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  1. 1.National Key Laboratory of Crop Genetic Improvement, Key Laboratory of Horticultural Plant Biology of Ministry of Education, College of Horticulture and Forestry SciencesHuazhong Agricultural UniversityWuhanP.R. China
  2. 2.National Institute of Fruit Tree ScienceTsukuba, IbarakiJapan