Biologia Plantarum

, Volume 55, Issue 1, pp 159–163

Effect of salt stress on gene expression of superoxide dismutases and copper chaperone in Arabidopsis thaliana

Brief Communication

DOI: 10.1007/s10535-011-0022-x

Cite this article as:
Attia, H., Karray, N., Msilini, N. et al. Biol Plant (2011) 55: 159. doi:10.1007/s10535-011-0022-x


Arabidopsis thaliana plants (wild type accessions Col and N1438) were grown in nutrient solution for 34 d with or without 50 mM NaCl. Salt stress inhibited plant growth rate more in Col than in N1438 and a decrease in K+, Ca2+ and nitrogen contents was observed in both accessions. NaCl diminished accumulation of malate, fumarate and citrate only in Col accession. To measure the effect of NaCl on transcript level of superoxide dismutase (SOD) isoforms and copper chaperone for SOD genes, a semi-quantitative polymerase chain reaction (RT-PCR) method was developed using cDNA normalized against the EF1a gene in parallel with quantitative real time RT-PCR (Q-PCR) technique. Both methods gave the same results. The abundance of transcripts of the three genes coding for Cu/Zn-SOD responded similarly to NaCl in both accessions: CSD1 gene was overexpressed, and CSD2 and CSD3 genes were repressed. However, the genes coding for Fe-SOD (FSD1), Mn-SOD (MSD1) and Cu-chaperone for SOD (CCS) responded to NaCl differently in Col and N1438: the former gene was overexpressed in Col and repressed in N1438, and the opposite behaviour was observed for the latter two genes.

Additional key words

citratefumarateintraspecific variabilitymalatemineral nutritionRT-PCR



copper chaperone for SOD


quantitative real time RT-PCR


reactive oxygen species


reverse transcription polymerase chain reaction


superoxide dismutase

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  1. 1.Physiologie et Biochimie de la Tolérance au Sel des Plantes, Faculté des Sciences de TunisCampus UniversitaireTunis El ManarTunisia