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Endogenous brassinosteroids in wheat treated with 24-epibrassinolide

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Biologia Plantarum

Abstract

The aim of the study was to examine the effect of exogenous 24-epibrassinolide on its uptake and content of endogenous brassinosteroids in wheat seedlings. 24-Epibrassinolide was applied at two concentrations (0.1 and 2.0 μM) and in three different methods: by soaking seeds, by drenching and by spraying plants. Brassinosteroids were determined by high-performance liquid chromatography combined with electrospray mass spectrometry. Three important brassinosteroids, 24-epibrassinolide, brassinolide and castasterone, were detected in the wheat leaves, but their contents varied with leaf insertion and plant age. Increased 24-epibrassinolide content in the leaf tissue was found when this hormone was applied by soaking or drenching. Additionally the seed treatment influenced brassinosteroid balance in seedlings. The growth response of wheat seedlings treated with 24-epibrassinolide has been also investigated.

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Abbreviations

BR:

brassinosteroid

24-epiBL:

24-epibrassinolide

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Acknowledgements

This study was financed by the European Union CROPSTRESS Program, Project QLK5-CT-2002-30424, by Polish Government, Research Project 2P06A01928 (2005–2007) and by the Ministry of Education, Youth and Sports of the Czech Republic (MSM 6198959216). The authors would like to thank to Dr. Ondřej Novák for help with LC-MS measurements and Prof. Miroslav Strnad for a critical reading of the manuscript. We also would like to thank Prof. David Morris (U.K.) for the English editing of this manuscript.

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Authors and Affiliations

  1. Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek 21, PL-30239, Kraków, Poland

    A. Janeczko

  2. Laboratory of Growth Regulators, Palacký University, Šlechtitelů 11, CZ-78371, Olomouc, Czech Republic

    J. Swaczynová

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  1. A. Janeczko
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  2. J. Swaczynová
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Correspondence to A. Janeczko.

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Janeczko, A., Swaczynová, J. Endogenous brassinosteroids in wheat treated with 24-epibrassinolide. Biol Plant 54, 477–482 (2010). https://doi.org/10.1007/s10535-010-0084-1

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  • DOI: https://doi.org/10.1007/s10535-010-0084-1

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