Abstract
The present paper reports a protocol for minimum growth conservation of Drosophyllum lusitanicum (L.) Link. in vitro. Double-node cuttings were maintained for 4, 8 and 12 months at 5 or 25 °C in the dark. The effects of sucrose either alone at 5, 20, 30, 40 and 60 g dm−3 or at 20, 40 and 60 g dm−3 in combination with 20 g dm−3 mannitol, on survival and post-storage shoot multiplication efficiency were investigated. The cultures could effectively be conserved under minimum growth at 5 °C for 8 months on Murashige and Skoog’s medium supplemented with 60 g dm−3 sucrose, 20 g dm−3 mannitol and 0.91 µM zeatin. Following extended conservation, the cultures could be successfully regenerated into new shoots, and they were morphologically similar to those of non-stored controls.
Abbreviations
- MS:
-
Murashige and Skoog (1962) medium
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Gonçalves, S., Romano, A. In vitro minimum growth for conservation of Drosophyllum lusitanicum . Biol Plant 51, 795–798 (2007). https://doi.org/10.1007/s10535-007-0163-0
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DOI: https://doi.org/10.1007/s10535-007-0163-0