Brief Communication

Biologia Plantarum

, Volume 51, Issue 3, pp 556-558

Genome size stability in six medicinal plant species propagated in vitro

  • E. SliwinskaAffiliated withLaboratory of Molecular Biology and Cytometry, Department of Genetics and Plant Breeding, University of Technology and Life Sciences Email author 
  • , B. ThiemAffiliated withDepartment of Pharmaceutical Botany and Plant Biotechnology, K. Marcinkowski University of Medical Sciences

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Abstract

DNA content was estimated by flow cytometry in seedlings and in vitro clones of six species: Oenothera paradoxa, Inula verbascifolia ssp. aschersoniana, Rubus chamaemorus, Solidago virgaurea, S. graminifolia and Pueraria lobata. With the exception of P. lobata, there was no difference in genome sizes between seedlings and in vitro cultured plants from any species, indicative that they maintain their genetic stability during in vitro culture. This confirms the usefulness of tissue culture for production of certified plant material to obtain herbal medicines.

Additional key words

DNA content flow cytometry genetic fidelity micropropagation