Biologia Plantarum

, Volume 51, Issue 3, pp 556–558

Genome size stability in six medicinal plant species propagated in vitro

Brief Communication

DOI: 10.1007/s10535-007-0121-x

Cite this article as:
Sliwinska, E. & Thiem, B. Biol Plant (2007) 51: 556. doi:10.1007/s10535-007-0121-x

Abstract

DNA content was estimated by flow cytometry in seedlings and in vitro clones of six species: Oenothera paradoxa, Inula verbascifolia ssp. aschersoniana, Rubus chamaemorus, Solidago virgaurea, S. graminifolia and Pueraria lobata. With the exception of P. lobata, there was no difference in genome sizes between seedlings and in vitro cultured plants from any species, indicative that they maintain their genetic stability during in vitro culture. This confirms the usefulness of tissue culture for production of certified plant material to obtain herbal medicines.

Additional key words

DNA contentflow cytometrygenetic fidelitymicropropagation

Copyright information

© Institute of Experimental Botany, ASCR 2007

Authors and Affiliations

  1. 1.Laboratory of Molecular Biology and Cytometry, Department of Genetics and Plant BreedingUniversity of Technology and Life SciencesBydgoszczPoland
  2. 2.Department of Pharmaceutical Botany and Plant BiotechnologyK. Marcinkowski University of Medical SciencesPoznańPoland