Original Papers

Biologia Plantarum

, 51:229

First online:

Effects of freezing on plasma membrane H+-ATPase of the callus from Chorispora bungeana

  • J. M. WuAffiliated withKey Laboratory of Arid and Pasture Agroecology of Ministry of Education, Lanzhou University
  • , Z. G. ZhaoAffiliated withKey Laboratory of Arid and Pasture Agroecology of Ministry of Education, Lanzhou University
  • , H. XingAffiliated withCollege of Agronomy, Gansu Agriculture University
  • , H. P. GuoAffiliated withKey Laboratory of Arid and Pasture Agroecology of Ministry of Education, Lanzhou University
  • , W. X. LiAffiliated withKey Laboratory of Arid and Pasture Agroecology of Ministry of Education, Lanzhou University
  • , L. Z. AnAffiliated withKey Laboratory of Arid and Pasture Agroecology of Ministry of Education, Lanzhou UniversityCold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Science Email author 
  • , S. J. XuAffiliated withKey Laboratory of Arid and Pasture Agroecology of Ministry of Education, Lanzhou University
  • , T. ChenAffiliated withCold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Science

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Abstract

The influence of freezing treatment on plasma membrane (PM) H+-ATPase was investigated using plasma membrane vesicles isolated from calluses from Chorispora bungeana Fisch. & C.A. Mey. by the discontinuous sucrose gradient centrifugation. Freezing treatment (−4 °C) for 5 d resulted in significant increases in the ATPase activity and the activity of p-nitrophenyl phosphate (PNPP) hydrolysis, decreases in the Km for ATP hydrolysis and PNPP hydrolysis, and the shift of optimal pH from 6.5 to 7.0. Also, the activity PNPP hydrolysis was less sensitive to vanadate after freezing treatment compared to control, while the inhibition of ATP hydrolysis by hydroxylamine was more sensitive. In addition, freezing treatment also decreased the activation effects of trypsin on PNPP hydrolysis, but increased the activation effects of lysophosphatidylcholine on ATP hydrolysis. Taken together, these results suggested that PM H+-ATPase might play an important role during adaptation to freezing and enhancing the frost hardness in C. bungeana.

Additional key words

alpine plant kinase domain p-nitrophenyl phosphate phosphatase domain