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The response of the TonB-dependent transport network in Anabaena sp. PCC 7120 to cell density and metal availability

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Abstract

TonB dependent transporters (TBDT) are an essential protein family in bacteria involved in the uptake of a broad variety of molecules such as siderophore-chelated iron, which was the first described substrate. Meanwhile it is known that TBDTs are involved in the uptake of many metals, sugars and polypeptides. The action of TBDTs is regulated and energized by the plasma membrane anchored TonB, which is charged by a proton pump. The number of the genes coding for TBDTs varies in different species, which might reflect environmental adaptations or evolutionary variations of the system. For example, in the cyanobacterium Anabaena sp. PCC 7120 the large number of 22 genes coding for TBDTs has been identified and the expression of these genes has been explored in the absence of iron or copper as well as under nitrogen starvation. We describe the analysis of the expression of the TBDT genes and the according cytoplasmic-membrane localized components; the latter appear to have a lower degree of complexity in Anabaena sp. PCC 7120. This analysis unravels that the response is not sole dependent on the metal supply, but also on cell culture densities. In addition, we present a large group of FhuA-like genes which is expressed highest under standard conditions suggesting a function distinct from iron or copper transport. The genes are clustered according to the expression profile and the consequences for our understanding of the transport systems in Anabaena sp. PCC 7120 are discussed.

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Acknowledgments

We would like to thank Alexander Hahn, Mareike Rudolf and Maike Ruprecht for helpful discussions and technical support. We thank Stefan Simm for the cluster analysis. The work was supported by grants from Deutsche Forschungsgemeinschaft (DFG SCHL585/6–1) and the Cluster of Excellence ‘Macromolecular Complexes’.

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  1. Center of Membrane Proteomics, Cluster of Excellence “Macromolecular complexes”, Department of Biosciences, Molecular Cell Biology of Plants, Goethe-University, Max-von-Laue-Street 9, 60438, Frankfurt, Germany

    Mara Stevanovic, Christina Lehmann & Enrico Schleiff

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  1. Mara Stevanovic
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  2. Christina Lehmann
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  3. Enrico Schleiff
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Correspondence to Enrico Schleiff.

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Stevanovic, M., Lehmann, C. & Schleiff, E. The response of the TonB-dependent transport network in Anabaena sp. PCC 7120 to cell density and metal availability. Biometals 26, 549–560 (2013). https://doi.org/10.1007/s10534-013-9644-0

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