BioMetals

, Volume 24, Issue 6, pp 981–992

Mechanisms underlying the protective effect of zinc and selenium against cadmium-induced oxidative stress in zebrafish Danio rerio

Authors

    • Laboratoire de Biochimie et Toxicologie de l’Environnement (UR04AGR05)ISA
  • Lina Chouchene
    • Unité de Recherche: Génétique, Biodiversité et Valorisation des Bioressources, Institut Supérieure de Biotechnologie de Monastir
  • Khaled Said
    • Unité de Recherche: Génétique, Biodiversité et Valorisation des Bioressources, Institut Supérieure de Biotechnologie de Monastir
  • Abdelhamid Kerkeni
    • Département de Biophysique, Faculté de Médecine de MonastirUnité de Recherche: Eléments Traces, Radicaux Libres, Antioxydants, Pathologies Humaines et Environnement
  • Imed Messaoudi
    • Unité de Recherche: Génétique, Biodiversité et Valorisation des Bioressources, Institut Supérieure de Biotechnologie de Monastir
Article

DOI: 10.1007/s10534-011-9456-z

Cite this article as:
Banni, M., Chouchene, L., Said, K. et al. Biometals (2011) 24: 981. doi:10.1007/s10534-011-9456-z

Abstract

The present study was designed to elucidate the protective effect mechanism of Zinc (Zn) and Selenium (Se) on cadmium (Cd)-induced oxidative stress in zebrafish. For this purpose we investigate the response of oxidative stress markers, metallothionein accumulation and gene expression in liver and ovary of female zebrafish exposed to 0,4 mg/l Cd in water and supplemented with Zn (5 mg kg−1) and/or Se (2 mg kg−1) for 21 days in their diet. Liver and ovary Cd uptake was evaluated after the exposure period. Cd exposure significantly inhibited the antioxidant enzyme activities termed as catalase (CAT), superoxide dismutase (SOD) and glutathione peroxydase (GPx) and caused a pronounced malondialdehyde (MDA) accumulation in both organs. Co-administration of Zn and Se reversed the Cd-induced toxicity in liver and ovary measured as MDA accumulation. Interestingly, gene expression patterns of Cat, CuZnSod and Gpx were up-regulated when related enzymatic activities were altered. Zebrafish metallothionein transcripts (zMt) significantly decreased in tissues of fish supplemented with Zn and/or Se when compared to Cd-exposed fish. Our data would suggest that Zn and Se protective mechanism against Cd-induced oxidative stress is more depending on the correction of the proteins biological activities rather than on the transcriptional level of related genes.

Keywords

Oxidative stressGene expressionCdZnSeZebrafishProtective effect

Copyright information

© Springer Science+Business Media, LLC. 2011