Article

BioMetals

, Volume 24, Issue 3, pp 513-522

Iron acquisition with the natural siderophore enantiomers pyochelin and enantio-pyochelin in Pseudomonas species

  • Zeb A. YouardAffiliated withDépartement de Microbiologie Fondamentale, Université de Lausanne
  • , Nicolas WennerAffiliated withDépartement de Microbiologie Fondamentale, Université de Lausanne
  • , Cornelia ReimmannAffiliated withDépartement de Microbiologie Fondamentale, Université de Lausanne Email author 

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Abstract

The bacterial siderophore pyochelin is composed of salicylate and two cysteine-derived heterocycles, the second of which is modified by reduction and N-methylation during biosynthesis. In Pseudomonas aeruginosa, the first cysteine residue is converted to its D-isoform during thiazoline ring formation, whereas the second cysteine remains in its L-configuration. Stereochemistry is opposite in the Pseudomonas fluorescens siderophore enantio-pyochelin, in which the first ring originates from L-cysteine and the second ring from D-cysteine. Both siderophores promote growth of the producer organism during iron limitation and induce the expression of their biosynthesis genes by activating the transcriptional AraC-type regulator PchR. However, neither siderophore is functional as an iron carrier or as a transcriptional inducer in the other species, demonstrating that both processes are highly stereospecific. Stereospecificity of pyochelin/enantio-pyochelin-mediated iron uptake is ensured at two levels: (i) by the outer membrane siderophore receptors and (ii) by the cytosolic PchR regulators.

Keywords

Siderophore Pseudomonas Enantiomer Pyochelin Enantio-pyochelin Stereospecificity