Biometals

, Volume 19, Issue 5, pp 473–481

Cadmium accumulation and interactions with zinc, copper, and manganese, analysed by ICP-MS in a long-term Caco-2 TC7 cell model

Authors

    • Agence Française de Sécurité Sanitaire des Aliments (AFSSA)Unité Contaminants Inorganiques et Minéraux de l’Environnement (CIME) – Equipe Métaux Lourds et Eléments minéraux (MET)
  • Céline Huynh-Delerme
    • Laboratoire de Pharmacie-Toxicologie – Ecole Nationale Vétérinaire d’Alfort
  • Thierry Guérin
    • Agence Française de Sécurité Sanitaire des Aliments (AFSSA)Unité Contaminants Inorganiques et Minéraux de l’Environnement (CIME) – Equipe Métaux Lourds et Eléments minéraux (MET)
  • Hélène Huet
    • Laboratoire de Pharmacie-Toxicologie – Ecole Nationale Vétérinaire d’Alfort
  • Jean-Marc Frémy
    • Agence Française de Sécurité Sanitaire des Aliments (AFSSA)Unité Contaminants Inorganiques et Minéraux de l’Environnement (CIME) – Equipe Métaux Lourds et Eléments minéraux (MET)
  • Martine Kolf-Clauw
    • Ecole Nationale Vétérinaire de Toulouse
Article

DOI: 10.1007/s10534-005-5147-y

Cite this article as:
Noël, L., Huynh-Delerme, C., Guérin, T. et al. Biometals (2006) 19: 473. doi:10.1007/s10534-005-5147-y

Abstract

The influence of long-term exposure to cadmium (Cd) on essential minerals was investigated using a Caco-2 TC7 cells and a multi-analytical tool: microwave digestion and inductively coupled plasma mass spectrometry. Intracellular levels, effects on cadmium accumulation, distribution, and reference concentration ranges of the following elements were determined: Na, Mg, Ca, Cr, Fe, Mn, Co, Ni, Cu, Zn, Mo, and Cd. Results showed that Caco-2 TC7 cells incubated long-term with cadmium concentrations ranging from 0 to 10 μmol Cd/l for 5 weeks exhibited a significant increase in cadmium accumulation. Furthermore, this accumulation was more marked in cells exposed long-term to cadmium compared with controls, and that this exposure resulted in a significant accumulation of copper and zinc but not of the other elements measured. Interactions of Cd with three elements: zinc, copper, and manganese were particularly studied. Exposed to 30 μmol/l of the element, manganese showed the highest inhibition and copper the lowest on cadmium intracellular accumulation but Zn, Cu, and Mn behave differently in terms of their mutual competition with Cd. Indeed, increasing cadmium in the culture medium resulted in a gradual and significant increase in the accumulation of zinc. There was a significant decrease in manganese from 5 μmol Cd/l exposure, and no variation was observed with copper.

Keywords

cadmiumCaco-2 cellsin vitro absorptionICP-MSmicrowave digestiontrace metals

Abbreviation

AAS

Atomic absorption spectrometry

CRM

Certified reference material

PBS

Phosphate buffered saline without calcium and magnesium

DMEM

Dubelcco’s modified Eagle’s medium

Copyright information

© Springer 2006