Exposure to high fluoride concentration in drinking water will affect spermatogenesis and steroidogenesis in male albino rats Article Received: 30 November 2004 Accepted: 10 January 2005 DOI:
Cite this article as: Pushpalatha, T., Srinivas, M. & Sreenivasula Reddy, P. Biometals (2005) 18: 207. doi:10.1007/s10534-005-0336-2 Abstract
Sodium fluoride (NaF) administered orally to adult male rats at a dose level of 4.5 ppm and 9.0 ppm for 75 days caused significant decrease in the body weight, brain index and testicular index. A significant decrease in sperm count, sperm motility, sperm viability and sperm function (HOS positive) with increased sperm abnormalities was also observed in NaF-exposed male rats. The activity levels of testicular steroidogenic marker enzymes 3
β-hydroxysteroid dehydrogenase (3 β-HSD) and 17 β-hydroxysteroid dehydrogenase (17 β-HSD) were significantly decreased in NaF-treated rats indicating decreased steroidogenesis and in turn spermatogenesis in rats exposed to NaF. Keywords sodium fluoride sperm male rat testes steroidogenic marker enzymes References Agalokova, WI, Lapin, AV, Gusev, GP. 2000 Effects of fluoride and vanedate on K + transport across the erythrocyte membrane of Rana temporaria Membr Cell Biol 13 527 536 PubMed Google Scholar Barot, VV. 1998 Occurrence of endemic fluorosis in human population of North Gujurat, India: human health risk Bull Environ Contam Toxicol 61 303 310 CrossRef PubMed Google Scholar Belsy, MA, Moghissi, KS, Eliasson, R, Paulsen, CA, Callegos, AJ, Prasad, MRN. 1980Laboratory Manual for the Examination of Human Semen and Semen Cervical Mucus Interaction Press Concern Singapore Google Scholar Bergmeyer, HU 1974 β-hydroxysteroid dehydrogenase Bergmeyer, eds. Methods of Enzymatic Analysis Academic Press New York 447 489 Google Scholar Boese, SR, Mac Lean, DC, El-Mogazi, D. 1995 Effects of fluoride on chlorophyll and fluorescence in spinach Environ Pollut 89 203 208 CrossRef PubMed Google Scholar Chinoy, NJ, Rao, MV, Narayana, MV, Neelakanta, E. 1991 Microdose vasal injection of sodium fluoride in the rat Reprod Toxicol 5 505 512 CrossRef PubMed Google Scholar Chinoy, NJ, Sequeira, E. 1989 Effects of fluoride on the histoarchitecture of reproductive organs of the male mouse Reprod Toxicol 3 261 267 CrossRef PubMed Google Scholar Collins, TF, Sprando, RL, Black, TN, et al. 2001 Multigenerational evaluation of sodium fluoride in rats Food Chem Toxicol 39 601 613 CrossRef PubMed Google Scholar Davies, MT, Port, GR, Davison, AW. 1998 Effects of dietary and gaseous fluoride on the aphid Aphis fabae Environ Pollut 99 405 409 CrossRef PubMed Google Scholar Dogeroglu, T, Cicek, A, Kara, S. 2003 Short-term effects of hydrogen fluoride on Nicotiana tobaccum L J Environ Sci Health B 38 561 570 PubMed Google Scholar Freni, SC. 1994 Exposure to high fluoride concentrations in drinking water is associated with decreased birth rates J Toxicol Environ Health 42 109 121 PubMed Google Scholar Ghosh, D, Das Sarkar, S, Maiti, R, Jana, D, Das, UB. 2002 Testicular toxicity in sodium fluoride treated rats: association with oxidative stress Reprod Toxicol 16 385 390 CrossRef PubMed Google Scholar Goh, EH, Neff, AW. 2003 Effects of fluoride on Xenopus embryo development Food Chem Toxicol 41 1501 1508 CrossRef PubMed Google Scholar Groth, E. 1975 An evaluation of the potential for ecological damage by chronic low level environmental pollution by fluoride Fluoride 8 224 240 Google Scholar Hoffman, DJ, Pattee, OH, Wiemeyer, SN. 1985 Effects of fluoride on screech owl reproduction: teratological evaluation, growth, and blood chemistry in hatchlings Toxicol Lett 26 19 24 CrossRef PubMed Google Scholar Jeyendran, RS, Vander Ven, HH, Zaneveld, LID. 1992 The hypoosmatic swelling test: an update Arch Androl 29 105 116 PubMed Google Scholar Kumar, A, Susheela, AK. 1994 Ultrastructural studies of spermatogenesis in rabbit exposed to chronic fluoride toxicity Int J Fert Men Std 39 164 171 Google Scholar Kumar, A, Susheela, AK. 1995 Effects of chronic fluoride toxicity on the morphology of ductus epididymis and the maturation of spermatozoa of rabbit Int J Exp Pathol 76 1 11 PubMed Google Scholar Lamberg, M, Hausen, H, Vartiamen, T. 1997 Symptoms experienced during periods of actual and supposed water fluoridation Community Dent Oral Epidemol 25 291 295 Google Scholar Mac Leod, J, Gold, RZ. 1953 The male factor in fertility and infertility, VII: semen quality in relation to age and sexual activity Fertil Steril 4 194 199 PubMed Google Scholar Narayana, MV, Chinoy, NJ. 1994 Effect of fluoride on rat testicular steroidogenesis Fluoride 27 7 12 Google Scholar
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