, Volume 17, Issue 6, pp 647–654

Ferrichrome in Schizosaccharomyces pombe – an iron transport and iron storage compound


  • Markus Schrettl
    • Department of Molecular BiologyMedical University Innsbruck
  • Günther Winkelmann
    • A-6020 Innsbruck, Austria. Department of Microbiology & BiotechnologyUniversity of Tuebingen
  • Hubertus Haas
    • Department of Molecular BiologyMedical University Innsbruck

DOI: 10.1007/s10534-004-1230-z

Cite this article as:
Schrettl, M., Winkelmann, G. & Haas, H. Biometals (2004) 17: 647. doi:10.1007/s10534-004-1230-z


Schizosaccharomyces pombe has been assumed not to produce siderophores. Nevertheless, the genomic sequence of this fission yeast revealed the presence of siderophore biosynthetic genes for hydroxamates. Applying a bioassay based on an Aspergillus nidulans strain deficient in siderophore biosynthesis, and using reversed-phase HPLC and mass spectrometry analysis, we demonstrate that S. pombe excretes and accumulates intracellularly the hydroxamate-type siderophore ferrichrome. Under iron-limiting conditions, the cellular ferrichrome pool was present in the desferri-form, while under iron-rich conditions, in the ferri-form. In contrast to S. pombe, hydroxamate-type siderophores could not be detected in two other yeast species, Saccharomyces cerevisiae and Candida albicans.


Copyright information

© Kluwer Academic Publishers 2004