Biogeochemistry

, Volume 111, Issue 1, pp 41–55

SOM genesis: microbial biomass as a significant source

  • Anja Miltner
  • Petra Bombach
  • Burkhard Schmidt-Brücken
  • Matthias Kästner
Synthesis and Emerging Ideas

DOI: 10.1007/s10533-011-9658-z

Cite this article as:
Miltner, A., Bombach, P., Schmidt-Brücken, B. et al. Biogeochemistry (2012) 111: 41. doi:10.1007/s10533-011-9658-z

Abstract

Proper management of soil organic matter (SOM) is needed for maintaining soil fertility and for mitigation of the global increase in atmospheric CO2 concentrations and should be informed by knowledge about the sources, spatial organisation and stabilisation processes of SOM. Recently, microbial biomass residues (i.e. necromass) have been identified as a significant source of SOM. Here, we propose that cell wall envelopes of bacteria and fungi are stabilised in soil and contribute significantly to small-particulate SOM formation. This hypothesis is based on the mass balance of a soil incubation experiment with 13C-labelled bacterial cells and on the visualisation of the microbial residues by means of scanning electron microscopy (SEM). At the end of a 224-day incubation, 50% of the biomass-derived C remained in the soil, mainly in the non-living part of SOM (40% of the added biomass C). SEM micrographs only rarely showed intact cells. Instead, organic patchy fragments of 200–500 nm size were abundant and these fragments were associated with all stages of cell envelope decay and fragmentation. Similar fragments, developed on initially clean and sterile in situ microcosms during exposure to groundwater, provide clear evidence for their formation during microbial growth and surface colonisation. Microbial cell envelope fragments thus contribute significantly to SOM formation. This origin and the related macromolecular architecture of SOM are consistent with most observations on SOM, including the abundance of microbial-derived biomarkers, the low C/N ratio, the water repellency and the stabilisation of biomolecules, which in theory should be easily degradable.

Keywords

Soil organic matterHumic compoundsC turnoverMicrobial biomassBacterial cell wallsScanning electron microscopy

Supplementary material

10533_2011_9658_MOESM1_ESM.doc (26 kb)
Supplementary material 1 (DOC 25 kb)
10533_2011_9658_MOESM2_ESM.doc (1.1 mb)
Supplementary material 2 (DOC 1172 kb)

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Anja Miltner
    • 1
  • Petra Bombach
    • 2
  • Burkhard Schmidt-Brücken
    • 3
    • 4
  • Matthias Kästner
    • 1
  1. 1.UFZ – Helmholtz-Centre for Environmental Research, Department of Environmental BiotechnologyLeipzigGermany
  2. 2.UFZ – Helmholtz-Centre for Environmental Research, Department of Isotope BiogeochemistryLeipzigGermany
  3. 3.Institute of Material ScienceTechnische Universität DresdenDresdenGermany
  4. 4.Papiertechnische StiftungAbteilung Oberflächenveredelung – Funktionale OberflächenHeidenauGermany