Biodegradation

, Volume 20, Issue 5, pp 593–601

Microbial populations related to PAH biodegradation in an aged biostimulated creosote-contaminated soil

  • Salvador Lladó
  • Nuria Jiménez
  • Marc Viñas
  • Anna Maria Solanas
Original Paper

DOI: 10.1007/s10532-009-9247-1

Cite this article as:
Lladó, S., Jiménez, N., Viñas, M. et al. Biodegradation (2009) 20: 593. doi:10.1007/s10532-009-9247-1

Abstract

A previous bioremediation survey on a creosote-contaminated soil showed that aeration and optimal humidity promoted depletion of three-ringed polycyclic aromatic hydrocarbons (PAHs), but residual concentrations of four-ringed benzo(a)anthracene (B(a)A) and chrysene (Chry) remained. In order to explain the lack of further degradation of heavier PAHs such as four-ringed PAHs and to analyze the microbial population responsible for PAH biodegradation, a chemical and microbial molecular approach was used. Using a slurry incubation strategy, soil in liquid mineral medium with and without additional B(a)A and Chry was found to contain a powerful PAH-degrading microbial community that eliminated 89% and 53% of the added B(a)A and Chry, respectively. It is hypothesized that the lack of PAH bioavailability hampered their further biodegradation in the unspiked soil. According to the results of the culture-dependent and independent techniques Mycobacterium parmense, Pseudomonas mexicana, and Sphingobacterials group could control B(a)A and Chry degradation in combination with several microorganisms with secondary metabolic activity.

Keywords

Polycyclic aromatic hydrocarbonsBiodegradationBioavailabilityBioremediationPyreneChrysene16SrRNADGGE

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Salvador Lladó
    • 1
  • Nuria Jiménez
    • 1
  • Marc Viñas
    • 2
  • Anna Maria Solanas
    • 1
  1. 1.Department of MicrobiologyUniversity of BarcelonaBarcelonaSpain
  2. 2.GIRO Technological CentreMollet del VallesSpain