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Kinetic analysis of the inhibitory effect of trichloroethylene (TCE) on nitrification in cometabolic degradation

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Abstract

In this study, the inhibitory effect of TCE on nitrification process was investigated with an enriched nitrifier culture. TCE was found to be a competitive inhibitor of ammonia oxidation and the inhibition constant (K I ) was determined as 666–802 μg/l. The TCE affinity for the AMO enzyme was significantly higher than ammonium. The effect of TCE on ammonium utilization was evaluated with linearized plots of Monod equation (e.g., Lineweaver–Burk, Hanes–Woolf and Eadie–Hofstee plots) and non-linear least square regression (NLSR). No significant differences were found among these data evaluation methods in terms of kinetic parameters obtained.

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Abbreviations

I::

inhibitory (non-growth) substrate concentration, μg non-growth substrate/l

K I ::

inhibition constant of inhibitory (non-growth) substrate, μg non-growth substrate/l

K iu::

dissociation constant of enzyme-substrate-inhibitory substance (ESI) complex, μg non-growth substrate/l

\(K_{\rm S}^{\rm app}\)::

apparent half-saturation constant for growth substrate, mg substrate/l

K S::

half-saturation constant for growth substrate, mg growth substrate/l

q::

specific substrate utilization rate, mg substrate/g VSS h

\(q_{{\rm NH}_{4}\hbox {-}{\rm N}}\)::

specific ammonium utilization rate, mg NH4-N/g VSS h

q max::

maximum specific substrate utilization rate, mg substrate/g VSS h

\(q_{{\rm max, NH}_{4}\hbox {-}{\rm N}}\)::

maximum specific ammonium utilization rate, mg NH4-N/g VSS h

\(q_{{\rm max, NH}_{4}\hbox {-}{\rm N}}^{\rm app}\)::

apparent maximum specific ammonium utilization rate, mg NH4-N/g VSS h

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Acknowledgement

The financial supports of this study by TUBITAK (Project No: IÇTAG A038) and Research Fund of Bogazici University (Project No: B.A.P. 02Y101D) are gratefully acknowledged. We thank Bulent Mertoglu and Nuray Guler for FISH and DGGE analyses of sludge samples.

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Authors and Affiliations

  1. Department of Environmental Engineering, University of Marmara, Kuyubasi, Istanbul, Turkey

    Bilge Alpaslan Kocamemi

  2. Institute of Environmental Sciences, University of Bogazici, Bebek, Istanbul, Turkey

    Ferhan Çeçen

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  1. Bilge Alpaslan Kocamemi
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  2. Ferhan Çeçen
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Correspondence to Bilge Alpaslan Kocamemi.

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Alpaslan Kocamemi, B., Çeçen, F. Kinetic analysis of the inhibitory effect of trichloroethylene (TCE) on nitrification in cometabolic degradation. Biodegradation 18, 71–81 (2007). https://doi.org/10.1007/s10532-005-9037-3

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