The rise of the invasives and decline of the natives: insights revealed from adult populations of container-inhabiting Aedes mosquitoes (Diptera: Culicidae) in temperate North America
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- Rochlin, I., Gaugler, R., Williges, E. et al. Biol Invasions (2013) 15: 991. doi:10.1007/s10530-012-0345-3
Container-inhabiting Aedes mosquitoes are successful invaders and important arthropod-borne disease vectors worldwide. In North America, a subtropical assemblage containing introduced Aedes albopictus and Aedes aegypti and the native Aedes triseriatus have served as a model for investigating ecological interactions during invasions and focused on the outcomes at the larval stages. We report a hypothesis driven study of a more temperate container Aedes assemblage at the adult population level monitored in the state of New Jersey during a 9-year period. The invasive A. albopictus and Aedes japonicus abundances increased by a factor of two, whereas A. triseriatus abundance decreased by a factor of three. Spatiotemporal analysis indicated these trends were coincident especially in the areas invaded by A. albopictus, leading to partial displacement of A. triseriatus. Although the invasive species reached peak abundance in highly urbanized areas, the native species’ rate of decline was similar across the urbanization gradient. Higher winter temperatures and precipitation favored increased A. albopictus abundance suppressing A. triseriatus adult populations in turn, whereas A. japonicus abundance was promoted by summer precipitation. The results validate the conceptual framework developed for subtropical container Aedes and suggest that the current climatic trends will favor further spread of A. albopictus, amplifying public health concerns.
KeywordsCompetitive displacement Invasive species Interspecies competition Urbanization Public health
Container- or tree hole-inhabiting mosquitoes from the genus Aedes have been intensively studied over several decades for a variety of reasons. From an ecological perspective, these mosquitoes and their preferred habitat (artificial containers or tree holes) provide a simple system for studying complex problems of species interactions, trophic processes, and community structure (Juliano 1998, 2009; Ellis et al. 2006). From an invasion biology perspective, container Aedes represent a model for one of the most successful invasive species on the planet (Juliano 1998; Lounibos 2002). From a public health perspective, these mosquitos are of exceptional importance as vectors of rapidly spreading or “re-emerging” pathogens such as dengue, chikungunya, yellow fever, West Nile, and other arboviruses (reviewed in Lounibos 2002; Juliano and Lounibos 2005; Weaver and Reisen 2010).
Among container Aedes, the Asian tiger mosquito, Aedes albopictus (Skuse), has figured prominently as a research subject due to high invasion potential illustrated through global spread, competitive larval advantages over other container Aedes, and the ability to vector numerous pathogens (Lounibos 2002; Juliano and Lounibos 2005). Shortly after A. albopictus invaded North America in the 1980’s, it was predicted to partially displace the native tree hole mosquito Aedes triseriatus (Coquillett) (Livdahl and Willey 1991). However, the number of field investigations studying these interactions have been relatively small and only scant evidence of the predicted outcomes have been presented (Lounibos et al. 2001; Juliano 2009).
The subtropical container Aedes assemblage in Florida, which consists of A. albopictus, A. triseriatus, and another invasive species, Aedes aegypti (L.), is the best described (Ho et al. 1989; Edgerly et al. 1999; Lounibos et al. 2001; Ellis et al. 2006; Juliano 2009). However, A. aegypti is not found in much of the eastern USA, where the Asian bush mosquito Aedes japonicus (Theobald), a recently introduced species which has not been adequately characterized from an ecological standpoint, is a common member of a temperate container Aedes assemblage (Peyton et al. 1999). For both assemblages, competitive asymmetry among larval stages appears to be the main force driving container Aedes population dynamics (Juliano 2009). Although the mechanisms of such species interactions have been well investigated experimentally, the impacts of such interactions on populations in nature remains uncertain (Lounibos et al. 2001; Juliano 2009). This may be due to lack of data on effects on the adults of these species, despite the importance of such information for both ecological theory and public health.
While previous studies concentrated on larval interactions and were primarily conducted in warmer climates, our analyses focus on adult populations within a temperate environment, coinciding with the northernmost distribution of A. albopictus along the USA eastern seaboard (Farajollahi and Nelder 2009). Long-term population trends and large-scale spatial dynamics of adult A. albopictus, A. japonicus, and A. triseriatus assemblage in eastern USA were examined in view of the following hypotheses. (1) A. albopictus is a stronger larval competitor and would displace the native A. triseriatus with some exceptions, see hypothesis #3 (Livdahl and Willey 1991; Novak et al. 1993; Teng and Apperson 2000; Lounibos et al. 2001); (2) A. japonicus is an inferior larval competitor to A. albopictus and a superior larval competitor to A. triseriatus (Armistead et al. 2008; Andreadis and Wolfe 2010; Alto 2011) resulting in similarly detectable trends in the adult populations; (3) A. triseriatus displacement trends are context-dependent with urban areas experiencing sharper declines compared to less disturbed rural environments (Livdahl and Willey 1991, Lounibos et al. 2001); (4) interactions among these species will be dependent on climatic conditions, with warmer temperatures generally favoring larval A. albopictus (Hanson and Craig 1995; Teng and Apperson 2000; Armistead et al. 2008), translating into increased adult population growth for this species.
Materials and methods
Study area, duration, and data sources
Mosquitoes were collected at 4,230 total trap sites throughout the state of New Jersey, USA during 2002–2010. Dry ice-baited CDC light traps and gravid traps with grass infusion were employed from May through October during each year to collect female mosquitoes. Due to similar patterns, data from CDC light and gravid traps were combined to reduce potential bias and to increase the power of analysis.
The urban areas shapefile were obtained from the Census Bureau (www.census.gov/geo/www/tiger/tigerua/ua_tgr2k.html). The Census Bureau’s urban areas represent densely developed territory with a population of 50,000 or more and encompass residential, commercial, and other non-residential urban land uses. Outside of these areas, territory containing at least 2,500 but less than 50,000 people is also considered urbanized (i.e. urban clusters). Rural areas are defined as any territory outside urban areas. Climatological data and long term trends were obtained from the Office of the New Jersey State Climatologist at Rutgers University (http://climate.rutgers.edu/ stateclim_v1/data).
Summary statistics for the dataset used in this study
Number of female mosquitoes (mean ± SE) collected per trap night in each 10 × 10 grid cell
Total number of female mosquitoes collected
Number of trap nights
Number of trapping locations
1.57 ± 0.67
0.91 ± 0.12
2.16 ± 0.25
1.1 ± 0.2
2.19 ± 0.23
2.24 ± 0.32
1.36 ± 0.25
3.36 ± 0.5
1.86 ± 0.48
2.21 ± 0.43
2.5 ± 0.28
0.95 ± 0.12
4.09 ± 0.6
1.96 ± 0.31
0.79 ± 0.2
2.98 ± 0.32
2.18 ± 0.26
0.87 ± 0.39
4.9 ± 0.53
1.64 ± 0.27
0.49 ± 0.08
3.04 ± 0.45
4.33 ± 0.54
0.94 ± 0.14
4.07 ± 0.46
1.55 ± 0.22
0.44 ± 0.09
Multivariate analyses comparing the overall changes in Aedes species abundance and composition through time were performed using nonparametric methods in Permanova+/Primer v 6.0 statistical package (Primer-E Ltd, Ivybridge, UK). PERMANOVA is a nonparametric analog of MANOVA using a Bray-Curtis resemblance matrix. SIMPER (contributions of variables to similarity) was used to elucidate the role of individual species in contributing to the significant differences by PERMANOVA. If no significant differences in Aedes species abundance and composition were found, consecutive years were grouped into time periods representing early, middle, and later stages of A. albopictus and A. japonicus invasions. This analysis was followed by univariate Mann–Whitney and Chi-square tests comparing abundance and spatial occurrence, respectively, of individual Aedes species through three time periods of early, middle, and later stages of invasion.
To characterize the association between weather and adult container Aedes abundance, temperature and precipitation data were extracted for the preceding winter, spring, summer, and fall for each year in 2002–2010. Mosquito abundance for each species was then regressed against each independent climatic variable separately, followed by fitting a multivariate model including all statistically significant climatic variables. The most parsimonious model was selected using stepwise procedure in linear regression. Univariate tests and regression analyses were performed in SPSS v20.0 (IBM Corp, Armonk, NY). Multiple comparisons were Bonferroni adjusted.
Spatial analysis was performed by Spatial Analysis by Distance Indices (SADIE, http://www.rothamsted.ac.uk/pie/sadie/SADIE_home_page_1.htm) (Perry et al. 1999) following by visualization in ArcMap (ESRI, Redlands, CA). Since only integers are accepted by the software, average trap counts per cell/time period were rounded to zero decimal points. Values > 0 but < 0.5 were rounded to one, indicating presence. A cluster described a region of high trap counts and a gap describes a region of low trap counts at the 90 % confidence interval. The statistical significance of spatial association analysis between two species was assessed by a randomization test with a 75 % confidence interval used due to the overly stringent correction for spatial autocorrelation in the software.
Temporal patterns in the container Aedes adult populations
A. albopictus is a stronger larval competitor and would displace the native A. triseriatus
Temporal changes in container Aedes species abundance through different stages of A. albopictus, and A. japonicus invasions
Average trap night collection within a 10 × 10 km grid cell**
SIMPER contributions to significant differences***
A. japonicus is an inferior larval competitor to A. albopictus and a superior larval competitor to A. triseriatus, resulting in detectable trends in adult populations
Temporal changes in container Aedes production and spatial distribution through different stages of A. albopictus, and A. japonicus invasions
% Grid cells ***
% Grid cells
% grid cells
Spatial patterns in the container Aedes adult populations
A. albopictus was a stronger larval competitor and displaced the native A. triseriatus, detectable at the adult population level
A. japonicus was an inferior larval competitor to A. albopictus and a superior larval competitor to A. triseriatus, resulting in detectable trends in the adult populations
Adult container Aedes spatiotemporal trends along the urbanization gradient
A. triseriatus displacement trends were context-dependent with urban areas experiencing sharper declines compared to less disturbed rural environments
The effect of urbanization on container Aedes abundance through different stages of A. albopictus, and A. japonicus invasions
Degree of urbanization*
Aedes species abundance**
A. albopictus average (E, M, L)
A. japonicus average (E, M, L)
A. triseriatus average (E, M, L)
2.01 (0.38, 0.91, 3.11)
2.11 (1.15, 2.43, 2.13)
1.51 (2.73, 1.86, 1.00)
2.5 (1.10, 1.31, 3.53)
2.17 (0.80, 2.49, 2.28)
1.03 (2.71, 1.41, 0.43)
2.83 (1.30, 1.56, 3.96)
2.36 (0.99, 3.01, 2.35)
0.97 (1.46, 1.51, 0.52)
3.99 (3.75, 2.51, 4.87)
2.4 (0.61, 2.85, 2.54)
1.26 (1.66, 2.06, 0.72)
Effects of climatic factors on container Aedes adult population trends
Interactions among container Aedes were dependent on climatic conditions, with warmer temperatures generally favoring larval A. albopictus and growth of the adult populations of this species
Climatic predictors of container Aedes abundance
Linear regression models with significant independent variables (IV)**
Single IV model all years, 2002–2010
Single IV model year 2003–2010***
Multiple IV model***
No significant IV
(B = 0.467,
R2 = 0.567, p = 0.031)
(B = 0.605, p < 0.001)
(B = 1.056, p = 0.001)
R2 = 0.955
(B = 0.531, R2 = 0.458, p = 0.045)
(B = −0.446, R2 = 0.438, p = 0.052)
No significant IV
(B = 0.531, p = 0.045)
R2 = 0.458
No significant IV
(B = −0.230, R2 = 0.618, p = 0.021)
(B = −0.284, p < 0.002)
(B = −0.410, p = 0.021)
R2 = 0.880
Container Aedes assemblages serve as an influential model for studying interactions among the community members, allowing for hypothesis testing in laboratory and field settings (Juliano 2009). However, knowledge gaps concerning the lack of data on the effects of larval interactions on the adult populations, scant number of field studies examining those effects, and insufficient understanding of temperate container Aedes assemblage ecology remain unresolved (Lounibos et al. 2001; Juliano 2009). The aim of the present study, therefore, was to evaluate adult container Aedes population trends over large spatial and temporal scales in temperate North America within the framework of four hypotheses grounded in the experiments and observations at the larval stage.
The seminal study by (Livdahl and Willey 1991) predicted that introduced A. albopictus would competitively displace native A. triseriatus mosquitoes in most areas of sympatry, but a more stable coexistence would develop in tree-hole communities compared to artificial containers. Further investigations revealed that this outcome was context-dependent and density-mediated. The competitive advantage of A. albopictus was enhanced under scarce food resources (Novak et al. 1993; Teng and Apperson 2000), when the predatory mosquito Toxorhynchites rutilus (Dyar & Knab) was present (Lounibos et al. 2001), or with increased larval densities (Teng and Apperson 2000). This advantage over A. triseriatus has also persisted in a three species assemblage with A. aegypti and A. albopictus in Florida (Ho et al. 1989). However, the number of field investigations have been small (Juliano 2009) and the competitive asymmetry between A. albopictus and A. triseriatus less manifested, especially in pristine woodland environments (Lounibos et al. 2001). Reduced interspecific competition (Livdahl and Willey 1991), selective consumption of A. albopictus by predators (Griswold and Lounibos 2006), and the intraguild predation by A. triseriatus (Edgerly et al. 1999) might account for the persistence of this native species in tree holes leading to a related second hypothesis that A. triseriatus displacement would not be as severe in less disturbed and rural areas (Lounibos et al. 2001).
Our analyses confirmed that A. albopictus invasion was accompanied by significant reductions in A. triseriatus adult populations, and demonstrated that these trends coincided in time and space. The effect was not only limited to the reduction in mosquito production, but also indicated local displacement at the spatial scales used in this study. Compared to our 2002 baseline data, the spatial extent of A. albopictus presence doubled from 36 to 72 % of the study area, whereas that of A. triseriatus decreased by almost the same scale (89 versus 53 %). Moreover, these changes occurred at the locations where high A. triseriatus densities were found prior to increases in A. albopictus abundance, providing strong support for the first hypothesis of competitive exclusion. The observed reduction in A. triseriatus adult populations cannot be attributed to the potential confounding factors related to the climate change such as direct effects of warming temperatures on this species. Aedes triseriatus occupies an extensive geographic range in North America from Texas and Florida in the south to Quebec and Manitoba in the north (Darsie and Ward 2005) and is not expected to be directly impacted by the climatic conditions within the study area.
The hypothesis on competitive advantage of A. triseriatus in less disturbed rural areas was not supported by our data. Inherent differences in container Aedes abundance and composition existed along the urbanization gradient, however, relatively consistent through time with A. triseriatus experiencing similar rate of decline in urban or less disturbed rural areas. The more precipitous decline in A. triseriatus abundance in suburban (by a factor of 3–6 between 2002 and 2006–2010 time periods) rather than highly urbanized areas (by a factor of 2.5) might be explained by the greater rate of increase for A. albopictus densities in less urban areas. Interestingly, the rate of A. triseriatus decline in more rural areas where it was the most abundant was similar to that in highly urbanized areas, suggesting that this indigenous species may find its optimal habitat where it would coexist (albeit in lower numbers) with the invasive species (Juliano 1998), even in highly disturbed environments.
The decreased persistence of rural A. triseriatus might be attributed to several factors. Unlike Florida, where predators such as T. rutilus are an important component of the community assemblage (Ellis et al. 2006; Griswold and Lounibos 2006), the only container mosquito predator in New Jersey is T. rutilus septentrionalis (Coquillett), a temperate subspecies which occurs in lower densities and exhibits higher rates of cannibalism (Farajollahi et al. 2009; Bartlett-Healy et al. 2012). Additionally, the intraguild predation by A. triseriatus might not be significant in the field (Juliano and Lounibos 2005) as corroborated by the local extinctions of A. triseriatus identified in our dataset. However, these conclusions may be influenced by the large spatial scale used in the analysis and a finer spatial grain might provide a more nuanced picture.
In addition to A. albopictus, another invasive species A. japonicus has been present in our study area since the late 1990’s (Peyton et al. 1999). The third hypothesis, therefore, concerned the competitive disadvantage of the native species in the presence of a second invasive is supported by a small number of studies indicating A. triseriatus as an inferior larval competitor to A. japonicus (Andreadis and Wolfe 2010; Alto 2011). Our study found A. japonicus well-established in northern New Jersey, where A. triseriatus counts were depressed compared to the rest of the state in the 2002 baseline. While this pattern in the northern study area appeared relatively stable, southern and coastal New Jersey areas experienced rapid rates of increase in A. albopictus adult abundance in 2003–2010 with a corresponding decline in A. triseriatus densities. Thus, the stabilized A. triseriatus populations in the north became the new highs co-localizing with A. japonicus clusters. In comparison, the overlaps between high counts of either of these two species with those of A. albopictus were rare. It is uncertain whether this balance will be upset by further encroachment of A. albopictus into northern New Jersey. This species is a superior competitor to A. japonicus (Armistead et al. 2008), and, in our study, A. japonicus adult populations experienced small (but significant) declines both temporally during the later stages of A. albopictus invasion (2006–2010 time period) and spatially as a displacement of high density clusters along the coast.
The slow A. albopictus intrusion into the more northern A. japonicus dominated areas may be assisted by the climatic changes that have a direct effect on the invasive Aedes mosquitoes (Juliano and Lounibos 2005). Warmer temperatures generally favor A. albopictus (Ho et al. 1989; Hanson and Craig 1995; Teng and Apperson 2000; Juliano and Lounibos 2005; Unlu and Farajollahi 2012) and lower temperatures limit the northern range of this species (Hanson and Craig 1995; Unlu and Farajollahi 2012). Precipitation may also determine the outcome of competition between A. albopictus and other Aedes species (Costanzo et al. 2005). In our study, adult A. albopictus abundance was positively associated with both high winter temperature and precipitation, which had the opposite effect on adult A. triseriatus abundance. These inexplicable negative effects on the native species contradict previous findings (Hanson and Craig 1995), and are best explained by the influence of winter weather on the populations of the invasive species, which are inversely associated with those of the native species. Warmer temperature or substantial snow cover may facilitate overwintering A. albopictus egg survival (Hanson and Craig 1995), likely resulting in increased larval densities and leading to suppressed adult A. triseriatus populations in the summer. No such relationship was found for A. japonicus, a temperate species with northern origins, which was positively associated with summer precipitation in this study. Wet summer conditions (e.g. year 2009, Fig. 1) may enhance A. japonicus competitive advantage over A. albopictus similarly to the relationship between the latter species and the tropical A. aegypti in Florida (Costanzo et al. 2005). However, as indicated previously by Farajollahi and Nelder (2009), the recent climate trends of warmer and wetter winters but average summer precipitation appear to favor further expansion of A. albopictus northward within our study area.
Interspecies interactions and climate change are vital for understanding population dynamics of invasive container Aedes mosquitoes and the pathogens they carry, also underscoring the importance of a strong ecological perspective for public health (Juliano and Lounibos 2005). Aedes albopictus has a greater disease transmission potential (Lounibos 2002; Weaver and Reisen 2010) and abundance compared to A. triseriatus across their range in New Jersey, especially in highly urbanized areas. Increased competition with A. japonicus may also lead to higher arbovirus infection rates in A. albopictus (Alto et al. 2005). Many other questions regarding the role of predation, trait-and density-mediated effects not only on ecology, but also on epidemiology of container Aedes and the pathogens vectored by these mosquito species remain unanswered (Juliano 2009). The conceptual framework for community ecology of container Aedes was largely developed in the subtropics and based on larval stages. These insights were fully validated by our study at the adult population level and in a more temperate climate with a different community. Thus, the robustness of ecological theory will allow further inquiry into the remaining questions in basic and applied science of ecological interactions, invasion biology, and public health implications.
The invasion of a stronger larval competitor, the Asian tiger mosquito (A. albopictus) was accompanied by a steep decline in the adult populations of the native A. triseriatus. These trends of increased A. albopictus populations and diminished A. triseriatus populations coincided in time and co-localized spatially over a large geographic area across both urban and rural locales. The competitive advantage of A. albopictus could be attributed almost entirely to warmer winter temperatures. The invasion of A. albopictus had a negative effect, but to a lesser degree, on another exotic mosquito, A. japonicus in the areas occupied by both species. The native A. triseriatus maintained higher populations in areas dominated by A. japonicus, suggesting less intense interspecific competition between these two species. This study demonstrated for the first time that interspecific interactions among larval container inhabiting Aedes can have profound effects on adult populations of these species in nature. The implications of these findings underscore the importance of a strong ecological perspective in vector biology, mosquito-borne disease epidemiology, and public health.
We thank the New Jersey State Mosquito Control Commission and the intense dedication and efforts of personnel located within all 21 mosquito control programs in New Jersey and the Center for Vector Biology at Rutgers University.
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