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A novel major facilitator superfamily transporter in Penicillium digitatum (PdMFS2) is required for prochloraz resistance, conidiation and full virulence

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Abstract

Objectives

To clone a novel major facilitator superfamily (MFS, a large protein family with diverse physiological functions in all kingdoms) transporter gene, Pdmfs2, and characterize its function in Penicillium digitatum.

Results

A novel MFS transporter gene, Pdmfs2, was isolated from P. digitatum. The full-length DNA of Pdmfs2 had a 1590 bp ORF encoding a full-size MFS transporter with 529 amino acids. In a prochloraz-resistant strain (PdHS-F6), Pdmfs2 transcript level was up-regulated compared with the prochloraz-sensitive strain (PdHS-E3) and could be induced by 7 μg prochloraz/ml. The deletion of Pdmfs2 (ΔPdmfs2) in PdHS-F6 led to increased susceptibility to prochloraz and lower EC50 value (the concentration of prochloraz producing 50 % growth inhibition) compared with the PdHS-F6 or complementation strain (COPdmfs2). The ΔPdmfs2 strain was defective in conidia yield and virulence towards citrus fruits, while the complementation of Pdmfs2 could restore the phenotypic features to a large extent.

Conclusions

Pdmfs2 is the second MFS transporter gene in P. digitatum and is required for prochloraz resistance, conidiation and full virulence.

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Acknowledgments

This work was supported by the National Natural Science Foundations of China (NO. 31371893, 31071653).

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    Authors and Affiliations

    1. Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Science, Central China Normal University, 152 Luoyu Road, Wuhan, 430079, Hubei, People’s Republic of China

      Zhi Wu, Shengqiang Wang, Yongze Yuan, Tingfu Zhang, Jing Liu & Deli Liu

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    1. Zhi Wu
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    2. Shengqiang Wang
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    3. Yongze Yuan
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    4. Tingfu Zhang
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    5. Jing Liu
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    6. Deli Liu
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    Corresponding author

    Correspondence to Deli Liu.

    Additional information

    Zhi Wu and Shengqiang Wang have contributed equally to this work.

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    Wu, Z., Wang, S., Yuan, Y. et al. A novel major facilitator superfamily transporter in Penicillium digitatum (PdMFS2) is required for prochloraz resistance, conidiation and full virulence. Biotechnol Lett 38, 1349–1357 (2016). https://doi.org/10.1007/s10529-016-2113-4

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    • DOI: https://doi.org/10.1007/s10529-016-2113-4

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