Abstract
Objective
An oral lactococcal-based vaccine which haboured the haemagglutinin1 (HA1) antigen fused to nisP anchor protein for the purpose of surface displaying the HA1 antigen was developed against H1N1 virus.
Results
Recombinant L. lactis strains expressed HA1-nisP fusion proteins when induced with nisin, as confirmed through western blotting. However, immunofluorescense did not detect any surface-displayed proteins, suggesting that the protein was either unsuccessfully translocated or improperly displayed. Despite this, oral administration of recombinant L. lactis strains to BALB/c mice revealed that significant levels of anti-HA1 sIgA antibodies were detected in mice fecal suspension samples of mice group NZ9000 (pNZ:HN) when compared to the negative control NZ9000 (pNZ8048) group.
Conclusion
Specific anti-HA1 sIgA antibodies were locally produced and live recombinant lactococcal vaccine was able to elicit humoral response of BALB/c mice despite unsuccessful surface display of the HA1 epitope.
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Acknowledgments
This work was supported by MOSTI Science fund grant number 09-05-IFN-MEB-005. The first author is a recipient of Graduate Research Fellowship (GRF), Universiti Putra Malaysia. There are no conflict of interests to declare.
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Supplementary Table 1—Primers used in this study.
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Joan, S.S.X., Pui-Fong, J., Song, A.AL. et al. Oral vaccine of Lactococcus lactis harbouring pandemic H1N1 2009 haemagglutinin1 and nisP anchor fusion protein elevates anti-HA1 sIgA levels in mice. Biotechnol Lett 38, 793–799 (2016). https://doi.org/10.1007/s10529-016-2034-2
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DOI: https://doi.org/10.1007/s10529-016-2034-2