Biotechnology Letters

, Volume 36, Issue 11, pp 2193–2198

Biotechnological approach towards a highly efficient production of natural prostaglandins

Authors

  • J. C. Guder
    • Biochemical EngineeringDECHEMA Research Institute
  • M. Buchhaupt
    • Biochemical EngineeringDECHEMA Research Institute
  • I. Huth
    • Biochemical EngineeringDECHEMA Research Institute
  • A. Hannappel
    • Biochemical EngineeringDECHEMA Research Institute
  • N. Ferreirós
    • Pharmazentrum Frankfurt/ZAFES, Institute of Clinical PharmacologyGoethe-University
  • G. Geisslinger
    • Pharmazentrum Frankfurt/ZAFES, Institute of Clinical PharmacologyGoethe-University
    • Biochemical EngineeringDECHEMA Research Institute
Original Research Paper

DOI: 10.1007/s10529-014-1610-6

Cite this article as:
Guder, J.C., Buchhaupt, M., Huth, I. et al. Biotechnol Lett (2014) 36: 2193. doi:10.1007/s10529-014-1610-6
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Abstract

Prostaglandins (PGs) act as potent local hormones in nearly all tissues of the human body and are used for various medical applications. Heterologous expression of PG endoperoxide H-synthase from the alga, Gracilaria vermiculophylla, into E. coli and the application of this strain in biotransformation experiments resulted in a highly efficient conversion of arachidonic acid (ARA) yielding up to 130 mg natural PGs l−1 in a laboratory scale approach. Detailed analyses of the products and production kinetics were performed, confirming a rapid conversion of ARA to PGs.

Keywords

Arachidonic acidBiotransformationE. coliGracilaria vermiculophyllaProstaglandinProstaglandin endoperoxide H-synthase

Copyright information

© Springer Science+Business Media Dordrecht 2014