Biotechnology Letters

, Volume 36, Issue 9, pp 1743–1752

miR-155 regulates the proliferation and cell cycle of colorectal carcinoma cells by targeting E2F2

  • Tong Li
  • Jue Yang
  • Xiaobo Lv
  • Kunmei Liu
  • Chao Gao
  • Yingying Xing
  • Tao Xi
Original Research Paper

DOI: 10.1007/s10529-014-1540-3

Cite this article as:
Li, T., Yang, J., Lv, X. et al. Biotechnol Lett (2014) 36: 1743. doi:10.1007/s10529-014-1540-3
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Abstract

MicroRNAs play important roles in carcinogenesis by negatively regulating the expression of target genes. Here we explore the biological function of miR-155 and the underlying mechanism in colorectal carcinoma. We validate, for the first time, that E2F2 is a direct target of miR-155 using western blot and a luciferase reporter assay and that miR-155 regulates the proliferation and cell cycle of colorectal carcinoma cells by targeting E2F2 using siRNA technology. We also found, for the first, time that E2F2 acts as a tumor suppressor in colorectal carcinoma. Overall, miR-155 plays an important role in colorectal carcinoma tumorigenesis by negative regulation of its targets including E2F2 and may be a potential therapeutic target for colorectal carcinoma treatment.

Keywords

Cell cycle Colorectal carcinoma E2F2 MicroRNA miR-155 Proliferation Survivin 

Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Tong Li
    • 1
    • 2
  • Jue Yang
    • 1
    • 2
  • Xiaobo Lv
    • 1
    • 2
  • Kunmei Liu
    • 3
  • Chao Gao
    • 1
  • Yingying Xing
    • 1
    • 2
  • Tao Xi
    • 1
    • 2
  1. 1.School of Life Science and TechnologyChina Pharmaceutical UniversityNanjingPeople’s Republic of China
  2. 2.Jiangsu Key Laboratory of Carcinogenesis and InterventionChina Pharmaceutical UniversityNanjingPeople’s Republic of China
  3. 3.Ningxia Key Laboratory of Cerebrocranial DiseasesNingxia Medical UniversityYinchuanPeople’s Republic of China