Identification of a fatty acid ∆6-desaturase gene from the eicosapentaenoic acid-producing fungus Pythium splendens RBB-5
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- Zhang, R., Zhu, Y., Ren, L. et al. Biotechnol Lett (2013) 35: 431. doi:10.1007/s10529-012-1101-6
A new full-length cDNA (PsD6) putatively encoding a ∆6-desaturase was cloned from the eicosapentaenoic acid-producing fungus Pythium splendens RBB-5. PsD6 contained an open reading frame of 1380 bp encoding a protein of 459 amino acids. The deduced amino acid sequence showed high similarity to those of other ∆6-desaturases. A recombinant vector expressing PsD6 (pPIC3.5K-D6) was constructed and transformed into Pichia pastoris GS115. The heterologous expressed PsD6 in P. pastoris desaturated linoleic acid to γ-linolenic acid but not desaturated α-linolenic acid to stearidonic acid. The results indicated that PsD6 was a fatty acid ∆6-desaturase and it had a substrate specificity for linoleic acid.