Biotechnology Letters

, Volume 35, Issue 1, pp 39–45

In vitro selection of peptide aptamers with affinity to single-wall carbon nanotubes using a ribosome display

  • Zha Li
  • Takanori Uzawa
  • Takashi Tanaka
  • Akira Hida
  • Koji Ishibashi
  • Hiromichi Katakura
  • Eiry Kobatake
  • Yoshihiro Ito
Original Research Paper

DOI: 10.1007/s10529-012-1049-6

Cite this article as:
Li, Z., Uzawa, T., Tanaka, T. et al. Biotechnol Lett (2013) 35: 39. doi:10.1007/s10529-012-1049-6
  • 742 Downloads

Abstract

A ribosome display from a diverse random library was applied for selecting peptide aptamers with high binding affinity to single-wall carbon nanotubes (SWCNTs). The selected peptide aptamer bound to and solubilized SWCNTs more strongly than did the peptide aptamer selected by a phage display method reported previously, and more strongly than other commonly used organic surfactants. The fluorescence spectrum of this aptamer showed a red shift upon interaction with SWCNTs but circular dichroism spectroscopy did not show any significant difference between the presence or absence of SWCNT binding.

Keywords

AptamerIn vitro selectionPeptideRibosome displaySingle-wall carbon nanotubes

Supplementary material

10529_2012_1049_MOESM1_ESM.docx (13 kb)
Supplementary material 1 (DOCX 13 kb)

Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Zha Li
    • 1
    • 2
  • Takanori Uzawa
    • 1
  • Takashi Tanaka
    • 3
  • Akira Hida
    • 4
  • Koji Ishibashi
    • 4
  • Hiromichi Katakura
    • 3
  • Eiry Kobatake
    • 2
  • Yoshihiro Ito
    • 1
  1. 1.Nano Medical Engineering LaboratoryRIKEN Advanced Science InstituteWako-shiJapan
  2. 2.Department of Biological InformationGraduate School of Bioscience and Biotechnology, Tokyo Institute of TechnologyYokohama-shiJapan
  3. 3.Nanosystem Research Institute (NRI), National Institute of Advanced Industrial Science and Technology (AIST)Tsukuba-shiJapan
  4. 4.Advanced Device LaboratoryRIKEN Advanced Science InstituteWako-shiJapan