Biotechnology Letters

, Volume 34, Issue 8, pp 1579–1587

Down-regulation of miRNA-221 triggers osteogenic differentiation in human stem cells

  • Behnaz Bakhshandeh
  • Maryam Hafizi
  • Nasser Ghaemi
  • Masoud Soleimani
Original Research Paper

DOI: 10.1007/s10529-012-0934-3

Cite this article as:
Bakhshandeh, B., Hafizi, M., Ghaemi, N. et al. Biotechnol Lett (2012) 34: 1579. doi:10.1007/s10529-012-0934-3

Abstract

Despite interesting in silico evidence, the specific role of mir-221 in osteogenesis has not been studied. We evaluated the osteogenic induction of transient-transfected anti-mir-221 in human unrestricted somatic stem cells and human mesenchymal stem cells both transcriptionally and translationally. In transfected unrestricted somatic stem cells, transcriptions of some osteogenic markers were twice that of the control and translations of osteopontin and osteocalcin were increased from 9 to 39 % and from 0 to 21 %, respectively. Up-regulation of transcribed osteogenic markers in transfected mesenchymal stem cells were 50 times greater than controls while no significant change in translations were observed. Prior to these analyses, the authenticity of stem cells, their osteogenic differentiation and transfection efficiency were verified. Transient modulation of mir-221 therefore suggests a mechanism for rapid induction of osteogenesis as a useful strategy for cell-based therapy.

Keywords

Ectopic transfectionMesenchymal stem cellsmicroRNA-221Osteogenic differentiationUnrestricted somatic stem cells

Supplementary material

10529_2012_934_MOESM1_ESM.doc (32 kb)
Supplementary material 1 (DOC 32 kb)
10529_2012_934_MOESM2_ESM.doc (48 kb)
Supplementary material 2 (DOC 48 kb)
10529_2012_934_MOESM3_ESM.tif (793 kb)
Supplementary material 3 (TIFF 793 kb)
10529_2012_934_MOESM4_ESM.tif (3.7 mb)
Supplementary material 4 (TIFF 3777 kb)

Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Behnaz Bakhshandeh
    • 1
    • 2
  • Maryam Hafizi
    • 2
  • Nasser Ghaemi
    • 3
  • Masoud Soleimani
    • 4
  1. 1.Department of BiotechnologyCollege of Science, University of TehranTehranIran
  2. 2.Stem Cell Biology DepartmentStem Cell Technology Research CenterTehranIran
  3. 3.School of ChemistryCollege of Science, University of TehranTehranIran
  4. 4.Department of HematologyFaculty of Medical Sciences, Tarbiat Modares UniversityTehranIran