Biotechnology Letters

, Volume 34, Issue 6, pp 1003–1007

Carbon source pulsed feeding to attain high yield and high productivity in poly(3-hydroxybutyrate) (PHB) production from soybean oil using Cupriavidus necator

  • José Geraldo da Cruz Pradella
  • Jaciane Lutz Ienczak
  • Cecília Romero Delgado
  • Marilda Keico Taciro
Original Research Paper

DOI: 10.1007/s10529-012-0863-1

Cite this article as:
da Cruz Pradella, J.G., Ienczak, J.L., Delgado, C.R. et al. Biotechnol Lett (2012) 34: 1003. doi:10.1007/s10529-012-0863-1

Abstract

Poly(3-hydroxybutyrate) (PHB) biosynthesis from soybean oil by Cupriavidus necator was studied using a bench scale bioreactor. The highest cell concentration (83 g l−1) was achieved using soybean oil at 40 g l−1 and a pulse of the same concentration. The PHB content was 81% (w/w), PHB productivity was 2.5 g l−1 h−1, and the calculated Yp/s value was 0.85 g g−1. Growth limitation and the onset of PHB biosynthesis took place due to exhaustion of P, and probably also Cu, Ca, and Fe.

Keywords

Cupriavidus necatorFatty acidsPoly(3-hydroxybutyrate)Pulsed feedingUnlimited carbon source

Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • José Geraldo da Cruz Pradella
    • 1
  • Jaciane Lutz Ienczak
    • 1
  • Cecília Romero Delgado
    • 2
  • Marilda Keico Taciro
    • 3
  1. 1.Laboratório Nacional de Ciência e Tecnologia do BioetanolCentro Nacional de Pesquisa em Energia e MateriaisCampinasBrazil
  2. 2.Laboratório de Biotecnologia IndustrialInstituto de Pesquisas Tecnológicas do Estado de São PauloSão PauloBrazil
  3. 3.Departamento de MicrobiologiaInstituto de Ciências Biomédicas, Universidade de São PauloSão PauloBrazil