Carbon source pulsed feeding to attain high yield and high productivity in poly(3-hydroxybutyrate) (PHB) production from soybean oil using Cupriavidus necator
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- da Cruz Pradella, J.G., Ienczak, J.L., Delgado, C.R. et al. Biotechnol Lett (2012) 34: 1003. doi:10.1007/s10529-012-0863-1
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Poly(3-hydroxybutyrate) (PHB) biosynthesis from soybean oil by Cupriavidus necator was studied using a bench scale bioreactor. The highest cell concentration (83 g l−1) was achieved using soybean oil at 40 g l−1 and a pulse of the same concentration. The PHB content was 81% (w/w), PHB productivity was 2.5 g l−1 h−1, and the calculated Yp/s value was 0.85 g g−1. Growth limitation and the onset of PHB biosynthesis took place due to exhaustion of P, and probably also Cu, Ca, and Fe.