Biotechnology Letters

, Volume 34, Issue 3, pp 527–531

Small RNA RyhB as a potential tool used for metabolic engineering in Escherichia coli

  • Zhen Kang
  • Xinran Wang
  • Yikui Li
  • Qian Wang
  • Qingsheng Qi
Original Research Paper

DOI: 10.1007/s10529-011-0794-2

Cite this article as:
Kang, Z., Wang, X., Li, Y. et al. Biotechnol Lett (2012) 34: 527. doi:10.1007/s10529-011-0794-2

Abstract

Small RNA (RyhB) was overexpressed artificially using an arabinose-inducible system in Escherichia coli and resulted in more succinate (7-fold) accumulation, which suggested that RyhB had a strong effect on sdhCDAB genes. Acetate was also increased indicating that RyhB had a comprehensive influence on glucose central metabolism. RyhB might therefore be useful for metabolic engineering of E. coli.

Keywords

Escherichia coliMetabolic engineeringRyhBsRNA

Supplementary material

10529_2011_794_MOESM1_ESM.pdf (35 kb)
Supplementary material 1 (PDF 34 kb)

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Zhen Kang
    • 1
  • Xinran Wang
    • 1
  • Yikui Li
    • 1
  • Qian Wang
    • 2
  • Qingsheng Qi
    • 1
    • 2
  1. 1.State Key Laboratory of Microbial TechnologyShandong UniversityJinanPeople’s Republic of China
  2. 2.National Glycoengineering Research CenterShandong UniversityJinanPeople’s Republic of China