Biotechnology Letters

, Volume 34, Issue 2, pp 303–307

Cd2+ binds to Atx1 and affects the physical interaction between Atx1 and Ccc2 in Saccharomyces cerevisiae

  • Dong-Hyuk Heo
  • In-Joon Baek
  • Hyun-Jun Kang
  • Ji-Hyun Kim
  • Miwha Chang
  • Chang-Min Kang
  • Cheol-Won Yun
Original Research Paper

DOI: 10.1007/s10529-011-0763-9

Cite this article as:
Heo, DH., Baek, IJ., Kang, HJ. et al. Biotechnol Lett (2012) 34: 303. doi:10.1007/s10529-011-0763-9

Abstract

The ATX1 deletion strain of Saccharomyces cerevisiae is more resistant to Cd2+ than the wild-type. To investigate the function of Atx1 in Cd2+ toxicity, we used a metal-binding assay to study the interaction between Atx1 and Cd2+ in vitro. Using circular dichroism and two-hybrid analyses, we found that Atx1 can bind Cd2+ specifically and that Cd2+ binding to Atx1 affects the physical interaction between Atx1 and Ccc2. These results imply that Atx1 delivers Cd2+ to Ccc2 and that this delivery is, at least in part, responsible for Cd2+ toxicity in S. cerevisiae.

Keywords

Atx1 Cadmium Ccc2 Copper Saccharomyces cerevisiae 

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Dong-Hyuk Heo
    • 1
  • In-Joon Baek
    • 1
  • Hyun-Jun Kang
    • 1
  • Ji-Hyun Kim
    • 1
  • Miwha Chang
    • 1
  • Chang-Min Kang
    • 1
  • Cheol-Won Yun
    • 1
  1. 1.School of Life Sciences and BiotechnologyKorea UniversitySeoulRepublic of Korea

Personalised recommendations