Abstract
The genetic associations and differences of four protein fractions were investigated in Tibetan wild barley. Albumin, globulin and hordein contents were under genetic control probably via multiple genes/quantitative trait loci. A correlation analysis showed that globulin was significantly associated with albumin, glutelin and hordein, while hordein was closely correlated with glutelin. Forty-nine diversity array technology (DArT) markers, which were distributed over seven chromosomes, were associated with the protein fraction contents. Those DArT markers associated with hordein were the same as those associated with globulin and glutelin. Only five markers associated with hordein, globulin and glutelin were also associated with albumin. Most of the protein fraction contents are therefore controlled by same genes which may contribute to total protein content. The discovery of new markers associated with specific protein fractions could be used to detect genes controlling protein content in the barley germplasm.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Celus I, Brijs K, Delcour JA (2006) The effects of malting and mashing on barley protein extractability. J Cereal Sci 44:203–211
Hu VW, Addington A, Hyman A (2011) Novel autism subtype-dependent genetic variants are revealed by quantitative trait and subphenotype association analyses of published GWAS data. PLoS One. doi:10.1371/journal.pone.0019067
Jin XL, Cai SG, Han Y, Wang J, Wei K, Zhang GP (2011) Genetic variants of HvGlb1 in Tibetan annual wild barley and cultivated barley and their correlation with malt quality. J Cereal Sci 53:59–64
Liu RH, Meng JL (2003) MapDraw: a Microsoft Excel macro for drawing genetic linkage maps based on given genetic linkage data. Heraditas (Beijing) 25:317–321
Polanda JA, Bradbury PJ, Buckler ES, Nelson RJ (2010) Genome-wide nested association mapping of quantitative resistance to northern leaf blight in maize. Proc Natl Acad Sci USA 108:6893–6899
Qi JC, Zhang GP, Zhou MC (2006) Protein and hordein content in barley seeds as affected by nitrogen level and their relationship to beta-amylase activity. J Cereal Sci 43:102–107
Qiu L, Wu DZ, Ali S, Cai SG, Dai F, Jin XL, Wu FB, Zhang GP (2011) Evaluation of salinity tolerance and analysis of allelic function of HvHKT1 and HvHKT2 in Tibetan wild barley. Theor Appl Genet 122:695–703
Rasmusson DC, Glass RL (1965) Effectiveness of early generation selection for four quality characters in barley. Crop Sci 5:389–391
Rostoks N, Ramsay L, MacKenzie K, Cardle L, Bhat PR (2006) Recent history of artificial outcrossing facilitates whole-genome association mapping in elite inbred crop varieties. Proc Natl Acad Sci USA 103:18656–18661
Sharbel TF, Haubold B, Mitchell-Olds T (2000) Genetic isolation by distance in Arabidopsis thaliana: biogeography and postglacial colonization of Europe. Mol Ecol 9:2109–2118
Shewry PR, Franklin J, Parmar S, Smith SJ, Miflin BJ (1983) The effects of sulphur starvation on the amino acid and protein compositions of barley grain. J Cereal Sci 1:21–31
Šimih G, Sudar R, Lalih A, Jurkovih Z, Horvat D, Babih D (2007) Relationship between hordein proteins and malt quality in barley cultivars grown in Croatia. Cereal Res Commun 35:1487–1496
Wei K, Xue DW, Huang YZ, Jin XL, Wu FB, Zhang GP (2009) Genetic mapping of quantitative trait loci associated with β-amylase and limit dextrinase activities and β-glucan and protein fraction contents in barley. J Zhejiang Univ Sci B 10:839–846
Wenzl P, Li H, Carling J, Zhou M, Raman H, Paul E, Hearnden P, Maier C, Xia L, Caig V, Ovesna J, Cakir M, Poulsen D, Wang J, Raman R, Smith KP, Muehlbauer GJ, Chalmers KJ, Kleinhofs A, Huttner E, Kilian A (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits. BMC Genom 7:206
Yin C, Zhang GP, Wang JM, Chen JX (2002) Variation of beta-amylase activity in barley as affected by cultivar and environment and its relation to protein content and grain weight. J Cereal Sci 36:307–312
Acknowledgments
This research was supported by the National Natural Science Foundation of China (No.30800681, 30971719), the Ministry of Science and Technology of China (20092X08009-076B) and the Zhejiang Provincial Natural Science Foundation of China (Y3100044).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Jin, X., Wei, K. & Zhang, G. A genome-wide association analysis of quantitative trait loci for protein fraction content in Tibetan wild barley. Biotechnol Lett 34, 159–165 (2012). https://doi.org/10.1007/s10529-011-0736-z
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10529-011-0736-z