Biotechnology Letters

, 33:2297

Transgenic potato overproducing l-ascorbic acid resisted an increase in methylglyoxal under salinity stress via maintaining higher reduced glutathione level and glyoxalase enzyme activity

  • Chandrama Prakash Upadhyaya
  • Jelli Venkatesh
  • Mayank Anand Gururani
  • Leonid Asnin
  • Kavita Sharma
  • Hemavathi Ajappala
  • Se Won Park
Original Research Paper

DOI: 10.1007/s10529-011-0684-7

Cite this article as:
Upadhyaya, C.P., Venkatesh, J., Gururani, M.A. et al. Biotechnol Lett (2011) 33: 2297. doi:10.1007/s10529-011-0684-7

Abstract

Salt-tolerance was studied in transgenic potato. It was conferred by overexpression of ascorbate pathway enzyme (d-galacturonic acid reductase, GalUR). As genetic engineering of the GalUR gene in potato enhances its ascorbic acid content (l-AsA), and subsequently plants suffered minimal oxidative stress-induced damage, we now report on the comprehensive aptness of this engineering approach for enhanced salt tolerance in transgenic potato (Solanum tuberosum L. cv. Taedong Valley). Potatoes overexpressing GalUR grew and tuberized in continuous presence of 200 mM of NaCl. The transgenic plants maintained a higher reduced to oxidized glutathione (GSH:GSSG) ratio together with enhanced activity of glutathione dependent antioxidative and glyoxalase enzymes under salinity stress. The transgenics resisted an increase in methylglyoxal that increased radically in untransformed control plants under salinity stress. This is the first report of genetic engineering of ascorbate pathway gene in maintaining higher level of GSH homeostasis along with higher glyoxalase activity inhibiting the accumulation in methylglyoxal (a potent cytotoxic compound) under salt stress. These results suggested the engineering of ascorbate pathway enzymes as a major step towards developing salinity tolerant crop plants.

Keywords

Abiotic stressAscorbateTransgenicsAntioxidant enzymesReduced glutathioneMethylglyoxal

Abbreviations

ASH

Ascorbate

APx

Ascorbate peroxidase

GalUR

d-Galacturonic acid reductase

GPx

Glutathione peroxidase

GR

Glutathione reductase

Gly I

Glyoxalase I

Gly II

Glyoxalase II

MG

Methylglyoxal

NADP

Nicotinamide adenine dinucleotide phosphate

GSSG

Oxidized glutathione

GSH

Reduced glutathione

ROS

Reactive oxygen species

UT

Untransformed control

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Chandrama Prakash Upadhyaya
    • 1
    • 2
  • Jelli Venkatesh
    • 1
  • Mayank Anand Gururani
    • 1
  • Leonid Asnin
    • 1
  • Kavita Sharma
    • 1
  • Hemavathi Ajappala
    • 1
  • Se Won Park
    • 1
  1. 1.Department of Molecular BiotechnologyKonkuk UniversityGwangjin-guKorea Republic
  2. 2.Department of BotanyGuru Ghasidas Central UniversityBilaspurIndia