Biotechnology Letters

, Volume 33, Issue 5, pp 897–902

TubeSpin bioreactor 50 for the high-density cultivation of Sf-9 insect cells in suspension

  • Qiuling Xie
  • Patrik O. Michel
  • Lucia Baldi
  • David L. Hacker
  • Xiaowei Zhang
  • Florian M. Wurm
Original Research Paper

DOI: 10.1007/s10529-011-0527-6

Cite this article as:
Xie, Q., Michel, P.O., Baldi, L. et al. Biotechnol Lett (2011) 33: 897. doi:10.1007/s10529-011-0527-6

Abstract

Here we present the TubeSpin bioreactor 50 (TubeSpins) as a simple and disposable culture system for Sf-9 insect cells in suspension. Sf-9 cells had substantially better growth in TubeSpins than in spinner flasks. After inoculation with 106 cells/ml, maximal cell densities of 16 × 106 and 6 × 106 cells/ml were reached in TubeSpins and spinner flasks, respectively. In addition the cell viability in these batch cultures remained above 90% for 10 days in TubeSpins but only for 4 days in spinner flasks. Inoculation at even higher cell densities reduced the duration of the lag phase. After inoculation at 2.5 × 106 cells/ml, the culture reached the maximum cell density within 3 days instead of 7 days as observed for inoculation with 106 cells/ml. Infection of Sf-9 cells in TubeSpins or spinner flasks with a recombinant baculovirus coding for green fluorescent protein (GFP) resulted in similar GFP-specific fluorescence levels. TubeSpins are thus an attractive option for the small-scale cultivation of Sf-9 cells in suspension and for baculovirus-mediated recombinant protein production.

Keywords

Baculovirus vector Bioreactor Sf-9 cells Spinner flask Suspension culture TubeSpin bioreactor 50 

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Qiuling Xie
    • 1
    • 2
  • Patrik O. Michel
    • 1
  • Lucia Baldi
    • 1
  • David L. Hacker
    • 1
  • Xiaowei Zhang
    • 1
    • 3
  • Florian M. Wurm
    • 1
  1. 1.Laboratory of Cellular Biotechnology, Institute of Bioengineering, École Polytechnique Fédérale de LausanneLausanneSwitzerland
  2. 2.Institute of Bioengineering, Jinan UniversityGuangzhouPeople’s Republic of China
  3. 3.College of Translational Medicine, Nanchang UniversityNanchangPeople’s Republic of China

Personalised recommendations