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Increase of ethanol tolerance of Saccharomyces cerevisiae by error-prone whole genome amplification

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Abstract

Saccharomyces cerevisiae was transformed for higher ethanol tolerance by error-prone whole genome amplification. The resulting PCR products were transformed back to the parental strain for homologous recombination to create a library of mutants with the perturbed genomic networks. A few rounds of transformation led to the isolation of mutants that grew in 9% (v/v) ethanol and 100 g glucose l−1 compared to untransformed yeast which grew only at 6% (v/v) ethanol and 100 g glucose l−1.

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References

  • Alper H, Moxley J, Nevoigt E, Fink GR, Stephanopoulos G (2006) Engineering yeast transcription machinery for improved ethanol tolerance and production. Science 314:1565–1568

    Article  PubMed  CAS  Google Scholar 

  • Crameri A, Raillard SA, Bermudez E, Stemmer WP (1998) DNA shuffling of a family of genes from diverse species accelerates directed evolution. Nature 39:288–291

    Google Scholar 

  • Lee KJ, Tribe DE, Rogers PL (1979) Ethanol production by Zymomonas mobilis in continuous culture at high glucose concentration. Biotechnol Lett 1:421–426

    Article  CAS  Google Scholar 

  • Patnaik R (2008) Engineering complex phenotypes in industrial strains. Biotechnol Prog 24:38–47

    Article  PubMed  CAS  Google Scholar 

  • Stouthamer AH, Bettenhaussen C (1973) Utilization of energy for growth and maintenance in continuous and batch cultures of microorganisms. A reevaluation of the method for the determination of ATP production by measuring molar growth yields. Biochim Biophys Acta 301:53–70

    PubMed  CAS  Google Scholar 

  • Xu B, Jin Z, Wang H, Jin Q, Jin X, Cen P (2008) Evolution of Streptomyces pristinaespiralis for resistance and production of pristinamycin by genome shuffling. Appl Microbiol Biotechnol 80:261–267

    Article  PubMed  CAS  Google Scholar 

  • Zhang L, Cui X, Schmitt K, Hubert R, Navidi W (1992) Whole genome amplification from a single cell: implications for genetic analysis. Proc Natl Acad Sci USA 89:5847–5851

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

We thank the Industrial Biotechnology Group members for their feedback and suggestions. This work was funded by Agency for Science, Technology and Research (A*STAR) in Singapore ICES/07-173B01.

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Correspondence to Hua Zhao.

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Luhe, A.L., Tan, L., Wu, J. et al. Increase of ethanol tolerance of Saccharomyces cerevisiae by error-prone whole genome amplification. Biotechnol Lett 33, 1007–1011 (2011). https://doi.org/10.1007/s10529-011-0518-7

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  • DOI: https://doi.org/10.1007/s10529-011-0518-7

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