, Volume 33, Issue 5, pp 1007-1011
Date: 19 Jan 2011

Increase of ethanol tolerance of Saccharomyces cerevisiae by error-prone whole genome amplification

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Abstract

Saccharomyces cerevisiae was transformed for higher ethanol tolerance by error-prone whole genome amplification. The resulting PCR products were transformed back to the parental strain for homologous recombination to create a library of mutants with the perturbed genomic networks. A few rounds of transformation led to the isolation of mutants that grew in 9% (v/v) ethanol and 100 g glucose l−1 compared to untransformed yeast which grew only at 6% (v/v) ethanol and 100 g glucose l−1.