Biotechnology Letters

, Volume 33, Issue 2, pp 403–409

Over-expression of microRNA169 confers enhanced drought tolerance to tomato

  • Xiaohui Zhang
  • Zhe Zou
  • Pengjuan Gong
  • Junhong Zhang
  • Khurram Ziaf
  • Hanxia Li
  • Fangming Xiao
  • Zhibiao Ye
Original Research Paper

DOI: 10.1007/s10529-010-0436-0

Cite this article as:
Zhang, X., Zou, Z., Gong, P. et al. Biotechnol Lett (2011) 33: 403. doi:10.1007/s10529-010-0436-0
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Abstract

Plant miRNA regulates multiple developmental and physiological processes, including drought responses. We found that the accumulation of Sly-miR169 in tomato (Solanum lycopersicum) was induced by drought stress. Consequently, Sly-miR169 targets, namely, three nuclear factor Y subunit genes (SlNF-YA1/2/3) and one multidrug resistance-associated protein gene (SlMRP1), were significantly down-regulated by drought stress. Constitutive over-expression of a miR169 family member, Sly-miR169c, in tomato plant can efficiently down-regulate the transcripts of the target genes. Compared with non-transgenic plants, transgenic plants over-expressing Sly-miR169c displayed reduced stomatal opening, decreased transpiration rate, lowered leaf water loss, and enhanced drought tolerance. Our study is the first to provide evidence that the Sly-miR169c negatively regulates stomatal movement in tomato drought responses.

Keywords

Drought tolerancemicroRNA169Stomatal openingTomato

Supplementary material

10529_2010_436_MOESM1_ESM.pdf (182 kb)
Supplementary material 1 (PDF 182 kb)

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Xiaohui Zhang
    • 1
  • Zhe Zou
    • 1
  • Pengjuan Gong
    • 2
  • Junhong Zhang
    • 2
  • Khurram Ziaf
    • 1
  • Hanxia Li
    • 2
  • Fangming Xiao
    • 3
  • Zhibiao Ye
    • 1
    • 2
  1. 1.National Key Laboratory of Crop Genetic ImprovementHuazhong Agricultural UniversityWuhanChina
  2. 2.Key Laboratory of Horticultural Plant Biology, Ministry of EducationHuazhong Agricultural UniversityWuhanChina
  3. 3.Department of Microbiology, Molecular Biology and BiochemistryUniversity of IdahoMoscowUSA